Screening Novel Drugresistant Mutations in Mycobacterium tuberculosis Strains by Using the Amplicon Sequencing Method
10.13865/j.cnki.cjbmb.2022.01.1510
- Author:
Peng-Peng YANG
1
;
Juan-Xiu LUO
1
;
Yu-Zhu SONG
1
;
Xue-Shan XIA
1
;
A-Mei ZHANG
1
;
Xiao-Fei LI
2
Author Information
1. Faculty of Life Science and Technology, Kunming University of Science and Technology
2. Department of Clinical laboratory, The Third People’ s Hospital of Kunming City
- Publication Type:Journal Article
- Keywords:
amplicon sequencing;
drug-resistant TB;
potential drug-resistant mutations
- From:
Chinese Journal of Biochemistry and Molecular Biology
2022;38(4):527-536
- CountryChina
- Language:Chinese
-
Abstract:
Tuberculosis is one of the seriously public problems. The increasing drug-resistant tuberculosis is the key problem for controlling tuberculosis. Rapid and accurate diagnosis is important for further treatment. In this study‚ a next-generation sequencing method based on amplicon sequencing was constructed to screen the mutations in 17 drug-resistant genes of five first-line anti-tuberculosis drugs. A total of 65 mutations were identified in 26 clinic drug-resistant tuberculosis strains‚ including 33 hotspot mutations‚ 9 rare mutations‚ and 23 novel mutations. The pathogenesis‚ conservation‚ and partial structures caused by 18 novel missense mutations were predicted. The results showed that 14 novel mutations showed high conservation in nine species. All these 14 mutations could change the partial structure of protein. According to the detection and analysis results of this study‚ it is speculated that these newly discovered mutations may be potential drug-resistant mutations. It is a rapid‚ accurate and comprehensive method for the detection of drug-resistant mutations in first-line drug-resistant Mycobacterium tuberculosis‚ which could identify hotspot and rare mutations together with novel mutations. The detection method may be used for clinical diagnosis and basic research.
