Effects of picropodophyllin on proliferation and cell cycle of human colorectal cancer HCT-15 cells and its mechanism
10.16098/j.issn.0529-1356.2020.02.014
- Author:
Li-Li ZHOU
1
;
Yang ZHAO
1
;
Sai-Xuan LIU
1
;
Xing-Bin SHEN
1
;
Rui-Ting WANG
2
;
Jing XUE
3
Author Information
1. Department of Pathology, The Affiliated Hospital of Chengde Medical University
2. Department of Pharmacology, Chengde Medical University
3. Experiment Center for Morphology, Chengde Medical University
- Publication Type:Journal Article
- Keywords:
Colorectal cancer cells HCT-15;
Flow cytometry;
Human;
Picropodophyllin;
Western blotting
- From:
Acta Anatomica Sinica
2020;51(2):233-238
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of picropodophyllin (PPP) on proliferation and cell cycle of human colorectal cancer cell line HCT-15 and to clarify the related mechanism. Methods Different concentrations of PPP were used to treat HCT-15 cells, the proliferative activity of HCT-15 cells was detected by cell courting kit-8(CCK-8). Morphological changes of HCT-15 cells were observed under inverted microscope. Flow cytometry was used to detect the changes of HCT-15 cell cycle and Western blotting was used to detect the expression of proliferating cell nuclear antigen (PCNA) and cell cyclinD1. Results The result of CCK-8 assay showed that PPP inhibited the proliferation of HCT-15 cells in a dose-time-dependent manner. Under the inverted microscope, it was found that the HCT-15 cells lost their original shape and became round, the refractive index decreased, and the number of living cells decreased significantly. Flow cytometry showed that the proportion of G
