Transfection of microRNA-21 mediating the biological behavior and cellular radiosensitivity of esophageal squamous carcinoma cells
10.16098/j.issn.0529-1356.2020.03.012
- Author:
Zhi-Xiong SHAO
1
;
Jun-Jie XIE
1
;
Zhen-Hua XU
1
;
Cheng ZENG
1
;
Qing-Ling SONG
1
;
Feng-Tao YI
1
Author Information
1. Department of Tumor Radiotherapy, Central Theater General Hospital of the Chinese People’s Liberation Army
- Publication Type:Journal Article
- Keywords:
Esophageal squamous cell;
Flow cytometry;
Invasion;
MicroRNA;
Proliferation;
Radiosensitivity
- From:
Acta Anatomica Sinica
2020;51(3):385-391
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the biological properties of microRNA (miRNA) -21 expression in esophageal squamous cell carcinoma. Methods MiRNA-21 sense expression vector, miRNA-21 empty vector and miRNA-21 inhibitor were transfected and detected by fluorescence Real-time PCR. The normal cells of esophageal squamous cell carcinoma TE-1 were used as the control group, and the transfection result were detected. The cell proliferation activity was detected by cell counting kit-8(CCK-8), and the apoptosis ability was detected by flow cytometry. The invasion ability and migration ability of TE-1 cells were observed by Transwell chamber and scratch test. Changes in the sensitivity of TE-1 cells to radiotherapy were observed by colony formation experiments. The data were analyzed using SPSS 19. 0 software. Results The result of Real-time PCR showed that the expression level of miRNA-21 in TE-1 cells was significantly decreased after transfected with miRNA-21 inhibitor (P<0. 05). Compared with the normal cell growth group, positive control group and negative control group, the proliferation of TE-1 cells was reduced and apoptosis was accelerated after transfection with miRNA-21 inhibitor (P<0. 05). In addition, the ability of cell invasion and migration in the experimental inhibition group was decreased. Clone formation assays showed that inhibition of miRNA-21 expression significantly increased the sensitivity of TE-1 cells to radiation. Conclusion Down-regulation of miRNA-21 reduces the proliferation, invasion, migration and sensitivity of radiotherapy in esophageal squamous cell carcinoma TE-1 cells, and is a potential target for the future treatment of esophageal cancer.
