Role of fibroblast growth factor 2 and tanshinoneⅡA in the differentiation of rat bone marrow mesenchymal stem cells into cardiomyocyte￣like cells
10.16098/j.issn.0529-1356.2020.04.008
- Author:
Jiao LI
1
;
Yang LÜ
1
;
Yang LIU
1
;
Hao-Yu WANG
1
;
Ya-Ru ZHAO
1
;
Xue-Jiao WANG
1
;
Hai-Ping WANG
1
Author Information
1. Department of Histology and Embryology, Hebei North University
- Publication Type:Journal Article
- Keywords:
Bone marrow mesenchymal stem cell;
Cardiomyocyte;
Fibroblast growth factor 2;
Flow cytometry;
Rat;
TanshinoneⅡA;
Western blotting
- From:
Acta Anatomica Sinica
2020;51(4):520-527
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of fibroblast growth factor 2 (FGF-2) combined with tanshinoneⅡA on differentiation of rat bone marrow mesenchymal stem cells (BMSCs) into cardiomyocyte-like cells. Methods BMSCs were isolated and cultured. The cultured cells were identified by flow cytometry, BMSCs were divided into experimental control group, FGF-2 group, tanshinoneⅡA group and the combined induction group. The activity and value of BMSCs were detected by MTT. The Real-time PCR method was used to detect BMSCs. Expression of early myocardial transcription factors GATA-4 and Nkx2. 5; Immunocytochemical staining for detection of connexin43(Cx43)and cardiac troponin-Ⅰ(cTnI); Immunofluorescence staining for detection of desmin and Tm; The expression of desmin and tropomyosin(Tm)was detected by Western blotting. Results The cell activity and proliferation after induction were good. The expression of GATA-4 and Nkx2. 5 in the induction group were stronger than that in the experimental control group, and the difference was statistically significant (P<0. 05). The positive expression of Cx43 and cTnI in the induction group increased significantly. The markers of the combined group were the most obvious, and the difference was statistically significant (P<0. 05). The expression of Myo-specific protein desmin and Tm in the combined induction group increased significantly, and the difference was statistically significant (P<0. 05). Conclusion Both FGF-2 and tanshinoneⅡA can promote the proliferation of BMSCs and induce the differentiation of BMSCs into cardiomyocyte-like cells. The synergistic effect of the two is better than other groups.
