Co-expression of plasmid-based protein kinase Bl-specific small interfering RNA and P53 synergistically inhibits proliferation, migration and invasion of hepatocellular carcinoma cells
10.16098/j.issn.0529-1356.2021.02.014
- Author:
Xiao-Long CHEN
1
;
Ping HUANG
1
;
Ya-Feng WANG
2
Author Information
1. Department of Hepatobiliary Surgery, The First Affiliated Hospital of Chongqing Medical University
2. Department of Hepatobiliary Surgery, Daping Hospital, Army Medical University
- Publication Type:Journal Article
- Keywords:
Dual expression plasmid;
Hepatocellular carcinoma;
Human;
P53;
Protein kinase B1;
Real-time PCR
- From:
Acta Anatomica Sinica
2021;52(2):251-257
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of the dual expression plasmid of protein kinase B1 (Akt 1)-specific siRNA and P53 on the proliferation, migration, invasion and apoptosis of hepatocellular carcinoma (HCC) cells. Methods We constructed a dual expression plasmid that co-expressed Akt 1-specific siRNA and wild-type p53 gene (pSi-Aktl-P53). The dual expression plasmid pSi-Aktl-P53 was transfected into HepG2 cells of HCC,The expression of Aktl and P53 was detected by Real-time PCR and Western blotting. Then, the dual expression plasmid was transfected into HepG2 cells, sh- Aktl plasmid and P53 plasmid were used as control. The effects of the dual plasmid on the proliferation, migration, invasion and apoptosis of HepG2 cells were detected by CCK-8 and 5-ethynyl-2'-deoxyuridine (EdU) experiments, Wound scratch experiment, Transwell chamber experiment and flow cytometry, respectively. Results After the dual plasmid was transfected into HepG2 cells, the expression of Aktl protein was significantly reduced and the expression of P53 protein was significantly increased in HepG2 cells. Compared with the shAktl and P53 plasmids, the dual expression plasmid pSi-Aktl-P53 significantly inhibited the proliferation N migration and invasion of HepG2 cells and significantly increased the apoptosis of HepG2 cells. Conclusion The dual expression plasmid pSi-Aktl-P53 can synergistically inhibit the proliferation, migration and invasion of HepG2 cells, significantly increased the apoptosis of HepG2 cells.
