Melatonin inhibiting the migration and invasion of gastric cancer cell line SGC-7901
10.16098/j.issn.0529-1356.2021.05.012
- Author:
Li XU
1
;
Xiu-Jiao CHEN
1
;
Chao CHEN
1
;
Wei-Nan ZHENG
1
;
Qing-Dong JIN
2
;
Rui-Xiang ZHOU
3
Author Information
1. Basic Medical College of Putian University
2. Department of Neurosurgery, Putian First Hospital
3. Department of Human Anatomy and Histology and Embryology, Neurobiology Research Center, Fujian Medical University
- Publication Type:Journal Article
- Keywords:
CD44;
Gastric cancer;
Intercellular cell adhesion molecule-1;
Matrix metalloproteinase-2;
Matrix metalloproteinase-9;
Melatonin;
P38;
Western blotting
- From:
Acta Anatomica Sinica
2021;52(5):751-758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the inhibitory effect of melatonin on the migration and invasion of gastric cancer cell and the underlying molecular mechanism. Methods Human gastric cancer SGC-7901 cells were treated with different concentrations of melatonin ( 0. 1, 1, 2 and 4 mmol/L ) for 24 hours, and the changes in the migration and invasion of gastric cancer cells were detected by scratch test and Transwell assay. The expressions of matrix metalloproteinase ( MMP) -2 and MMP-9 in the supernatant were detected by ELISA kit. The changes of MMP-2, MMP-9, intercellular cell adhesion molecule-1 ( ICAM-1 ) and CD44 expressions were detected by using Real-time PCR. The protein expressions of ICAM-1, CD44, p-P38, P38 and phosphorylated mitogen-activated protein kinase kinase ( p-MKK) 3/6 were detected by Western blotting. Results Melatonin inhibited the migration and invasion of gastric cancer cells in a dose- dependent manner. Compared with the blank control group, melatonin reduced the expression of MMP-2, MMP-9, ICAM-1 and CD44, and inhibited the expressions of p-P38, P38 and p-MKK3/6 in gastric cancer cells. Conclusion Melatonin inhibits the migration and invasion of gastric cancer cells by inhibiting the expressions of MMP-2, MMP-9, ICAM-1 and CD44. Inhibition may be related to the p38MAPK signaling pathway.
