Effect of Wnt5b overexpression on the differentiation of embryonic liver stem cells in mice and its repair effect on chronic liver injury in mice
10.16098/j.issn.0529-1356.2022.04.009
- Author:
Hao JIANG
1
;
Zhuo-Jun XIE
1
;
Hao-Dong ZHANG
1
;
Hong ZHOU
1
;
Qing LUO
1
;
Quan KANG
1
;
Quan KANG
2
Author Information
1. Department of of General and Trauma Surgery, Children’s Children’s Hospital of Chongqing Medical Chongqing Medical University, Ministry of Education Key Laboratory of Child Development and Disorders, National Clinical Research Center for Child Health and Disorders, China International Science and Technology Cooperation Base of Child Development and Critical Disorders, Chongqing Key Laboratory of Pediatrics
2. Department of Pediatric Reasearch Institute, Children’s Hospital of Chongqing Medical University
- Publication Type:Journal Article
- Keywords:
Chronic liver injury;
Fetal liver stem cell;
Liver fibrosis;
Mouse;
Real-time PCR;
Wingless-type MMTV integration site family member 5b
- From:
Acta Anatomica Sinica
2022;53(4):461-469
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective To investigate the effect of wingless-type MMTV integration site family member 5b(Wnt5b) gene overexpression mediated by recombinant adenovirus on the differentiation of mouse embryonic liver stem cells and repair of chronic liver injury in mice. Methods Recombinant adenoviruses expressing Wnt5b and green fluorescent protein (GFP) were applied respectively to infect mouse fetal liver stem cells HP14-19, and induced its differentiation and verified the expression of Wnt5b through Real-time PCR and Western blotting. It also applied indocyanine grean(ICG) uptake experiment and periodic acid-schiff(PAS) staining to detect the differentiation ability of HP14-19 into hepatocyte-like cells. The Real-time PCR was chosen to detect hepatocyte markers albumin (Alb) and cytokeratin 18 (CK18) expression. Forty-eight experimental male BALB/ c mice were randomly divided into control group, model group, stem cell treatment group and Wnt5b modified stem cell treatment group. The carbon tetrachloride(CCl
