Molecular analysis of interleukin 10 promoter region-592A/C polymorphism in hypertensive disorders of pregnancy in Qinghai Han nationality
10.16098/j.issn.0529-1356.2023.01.009
- Author:
Qian DUAN
1
;
Jian-Hua LI
1
;
Wen-Jia LI
1
;
Ru WANG
1
;
Zhen WU
1
;
Ming YUAN
1
Author Information
1. Department of Human Anatomy, Basic Medicine Science, Medical College of Qinghai University
- Publication Type:Journal Article
- Keywords:
Cytokine;
Genetic polymorphism;
Hypertensive disorders of pregnancy;
Interleukin 10;
Polymerase chain reaction-restriction fragment length polymorphism;
Pregnant woman;
Real-time PCR
- From:
Acta Anatomica Sinica
2023;54(1):63-69
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the association between the interleukin 10 (IL-10) gene promoter region-592A/C (rs1800872) polymorphism and hypertensive disorders of pregnancy (HDP) in Han women of Qinghai province and to determine the expression of this gene in two groups (HDP group and healthy control group) preliminarily. Methods A total of 140 HDP patients (HDP group) and 140 normal pregnant women (control group) in Qinghai Province were selected. Using blood DNA as template, the IL-10-592A/C polymorphism typing of HDP group and control group was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and verified by sequencing. The expression of IL-10 mRNA in the placental tissues of the two groups was detected by Real-time PCR. Plasma IL-10 levels of the two groups were detected by ELISA. Results The frequencies of AA, AC and CC genotypes of IL-10 gene in HDP group and control group were 24. 29%, 44. 29%, 31. 42% and 13. 57%, 41. 43%, 45. 00% respectively, the difference in genotype distribution between the two groups was statistically significant (P<0. 05);AA genotype frequency in HDP group(24. 29%)was higher than that of control group(13. 57%)(P<0. 05), CC genotype frequency in HDP group (31. 42%) was lower than that in control group (45. 00%) (P < 0. 05), while there was no significant difference in genotype frequency of AC between the two groups (P<0. 05); The distribution of A and C allele frequencies of IL-10592A/C polymorphism was different between the two groups, and the A allele frequency of HDP group was higher than that of control group (
