Effects of pranlinide on cognitive behavior, β amyloid protein 6E10, inflammatory factors and neuronal cell morphology in brain and retina of 5×FAD mice and WT mice
10.16098/j.issn.0529-1356.2023.03.005
- Author:
Yi-Wei HOU
1
;
Yu YANG
1
;
Bo WANG
1
;
Ying-Tao DUAN
1
;
Hong-Bo YAO
1
Author Information
1. 2018 Grade of Basic Medical Science College, Qiqihar Medical University
- Publication Type:Journal Article
- Keywords:
Alzheimer’s disease;
Enzyme-linked immunosorbent assay;
Interleukin-1β;
Mouse;
Pranlinide;
Tumor necrosis factor-α;
β amyloid protein 6E10
- From:
Acta Anatomica Sinica
2023;54(3):283-288
- CountryChina
- Language:Chinese
-
Abstract:
[Abstract] Objective To study the effects of pranlinide on cognitive behavior, β amyloid(Aβ) protein 6E10, inflammatory factors and neuronal cell morphology in brain and retina of 5×FAD mice and WT mice. Methods Thirty two 5×FAD mice and 16 WT mice were selected. All were female. 5×FAD mice were randomly divided into blank group and treatment group; No treatment was given in WT group. Blank group was intraperitoneally injected with PBS; treatment group was received intraperitoneal injection of pranlinide once a day for 8 weeks. The changes of cognitive ability were measured by Morris water maze test. The expression of Aβ6E10 protein in mice hippocampal cells and retina was detected by immunohistochemistry. Tumor necrosis factor α(NF-α) was determined by enzyme-linked immunosorbent assay. The same method was also used for interleukin-1β(IL-1β) detection (The content of inflammatory factors). The arrangement and morphology of nerve cells in mouse hippocampal tissue were determined by hematoxylin-eosin (HE) staining. Results The latency time of treatment group was shorter than that of 5×FAD group,and the times of crossing the platform and the percentage of target quadrant stay in the treatment group were higher than those in the 5×FAD group, and the differences were statistically significant (P<0. 05). Compared with the retinal area and the brain area of Aβ6E10 in the 5×FAD group and in the treatment group, the content was halved (P<0. 05). There was positive correlation between Aβ6E10 in retina and brain (P<0. 05).There was no significant difference in TNF-α and IL-1β in brain and retina between treatment group and 5×FAD group (P>0. 05). Compared with the 5×FAD group, the nerve cells in the treatment group were arramged in order and clear relatively. The distribution of glial cells was concentrated; The surrounding clearance was small. Conclusion Pranlinide can improve the cognitive ability of mice. The arrangement of nerve cells is regular, the shape is regular and the boundary is clear; The distribution of glial cells is concentrated; surrounding of clearance decrease. Aβ6E10 is synchronized in brain and retina.
