Effects of down-regulating lncRNA LINC00176 on cisplatin resistance and autophagy in lung cancer A549/DDP cells
10.12092/j.issn.1009-2501.2021.06.003
- Author:
Hua CUI
1
;
Mei YIN
1
;
Hongxia YAN
2
Author Information
1. Department of Respiratory and Critical Medicine, General Hospital of Ningxia Medical University
2. Department of Neurology, The Second People's Hospital of Yinchuan
- Publication Type:Journal Article
- Keywords:
Autophagy;
Cisplatin resistance;
LINC00176;
Lung cancer;
MiR-138-5p
- From:
Chinese Journal of Clinical Pharmacology and Therapeutics
2021;26(6):616-623
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To study the effects and mechanism of down-regulating lncRNA LINC00176 on cisplatin resistance and autophagy in lung cancer A549/DDP cells. METHODS: The qRT-PCR method was used to determine the expression changes of LINC00176 in normal bronchial epithelial 16HBE cells and lung cancer A549, A549/DDP, NCI-H1299 and SK-MES-1 cells. A549/DDP cells were divided into Control group, si-NC group, and si-LINC00176 group, si-LINC00176+ Anti-miR-NC group, and si-LINC00176+ Anti-miR-138-5p group. MTT experiment detected the half inhibitory concentration (IC
