ZNRF2 attenuates PC12 cell injury caused by oxygen-glucose deprivation and reoxygenation through inhibiting autophagy
10.3969/j.issn.1001-1978.2021.06.007
- Author:
Chao GU
1
;
Xiao-Dan TAN
1
;
Pu XIANG
1
;
Ying LUO
1
;
Jun-Qing YANG
1
;
Hong WANG
1
;
Chao GU
2
;
Xiao-Dan TAN
2
;
Ying LUO
2
;
Jun-Qing YANG
2
;
Hong WANG
2
;
Pu XIANG
3
Author Information
1. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, Chongqing Medical University
2. Dept of Pharmacology, Pharmacy School, Chongqing Medical University
3. Pharmacy Dept, Dianjiang People's Hospital of Chongqing
- Publication Type:Journal Article
- Keywords:
apoptosis;
autophagy;
mTOR;
oxygen-glucose deprivation/reoxygenation;
PC12 cells;
ZNRF2
- From:
Chinese Pharmacological Bulletin
2021;37(6):768-774
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the protective effect of ZN-RF2 on OGD/R-induced injury and the autophagy-related mechanism in PC12 cells. Methods PC12 cells were cultured in vitro and divided into normal group and OGD/R group. qRT-PCR and Western blot were used to measure the mRNA and protein expressions of ZNRF2. To explore the effect of ZNRF2 on OGD/R-induced injury in PC12 cells, cells were grouped into normal group, OGD/R group, LV-ZNRF2 group, LV-NC group, siR-ZNRF2 group and siNC group. Cell viability was detected by MTT assay, cell apoptosis was measured by flow cytometry and the expressions of autophagy-related proteins LC3II, p62, Beclin-l were accessed by Western blot. Results Compared with normal group, the cell viability decreased in OGD/R group, the cell apoptosis increased markedly, and the expressions of ZNRF2 mRNA and protein were downregulated significantly. Simultaneously, the proteins expressions of LC3II and Beclin-1 increased, and the expression of p62 protein decreased in OGD/R group. Compared with OGD/R group, the cell viability was enhanced, the cell apoptosis and autophagy were decreased in LV-ZNRF2 group. In contrast, the cell viability decreased and the cell apoptosis and autophagy were aggravated after transfecting siR-ZNRF2. Conclusions ZNRF2 protects PCI2 cells from the injury caused by OGD/R and its mechanism may be related to the inhibition of autophagy.
