Effect of MANF on proliferation, migration, invasion and apoptosis of colorectal cancer Caco-2 cells
10.3969/j.issn.1001-1978.2021.09.017
- Author:
Ying CHEN
1
;
Jie WANG
2
;
Chao HOU
2
;
Qian ZHAO
3
;
Qian-Qian NI
4
Author Information
1. School of Nursing, Anhui Medical University
2. School of Basic Medical Sciences, Anhui Medical University
3. Dept of Neonatology, The First Affiliated Hospital of Anhui Medical University
4. Dept of Obstetrics, The First Affiliated Hospital of USTC
- Publication Type:Journal Article
- Keywords:
apoptosis;
colorectal cancer;
invasion;
MANF;
migration;
NF-κB;
proliferation
- From:
Chinese Pharmacological Bulletin
2021;37(9):1286-1290
- CountryChina
- Language:Chinese
-
Abstract:
Aim To construct colorectal cancer Caco-2 cell line with overexpression of mesencephalic astrocyte-derived neurotrophic factor (MANF) and to investigate the effects of MANF on the proliferation, migration, invasion and apoptosis of human colorectal cancer Caco-2 cells. Methods Caco-2 cells were transfected with MANF-GFP plasmid and GFP empty vector plas-midrespectively. Real time polymerase chain reaction (RT-PCR) and Westernblot (WB) were used to detect the the expression level of MANF. Transwell was used to detect the effect of MANF on the migration and invasion ability of Caco-2 cells. CCK8 was used to dectet the influence of MANF on the proliferation of Caco-2 cells. WB and flow cytometrywere used to detect the activation ofcaspase-3andthe apoptosis rateof Caco-2 cells. Luciferase experiment was used to verify the effect of MANF on NF-κB pathway. Results MANF-GFP plasmid was successfully expressed in Caco-2 cells. The migration and invasion ability of Caco-2 cells were effectively reduced (P <0. 01), and the a-bility of the cell proliferation was downregulated (P < 0.01) in the MANF overexpression group; the expression of cleaved caspase-3 protein and apoptosis of Caco-2 cells were upregulated; compared with the control group, the transfection of MANF plasmid significantly reduced the transcription activity of NF-κB (P < 0. 01). Conclusions MANF can inhibit proliferation, migration, invasion of Caco-2 cells, while promote its apoptosis by negatively regulating NF-κB signal pathway.
