Effect of microRNA-125b on proliferation, apoptosis and collagen expression of hepatic stellate cells
10.3969/j.issn.1001-1978.2021.08.018
- Author:
Jia-Hui WANG
1
;
Lei WANG
1
;
Yang ZHENG
1
;
Yue PENG
2
;
Xian-Ling YUAN
2
;
Tie-Jian ZHAO
2
Author Information
1. Faculty of Chinese Medicine Science, Guangxi University of Traditional Chinese Medicine, Dept of Medicine
2. School of Basic Medicine, Guangxi University of Traditional Chinese Medicine, Dept of Physiology
- Publication Type:Journal Article
- Keywords:
apoptosis;
cell proliferation;
collagen;
hepatic stellate cells;
liver fibrosis;
miR-125 b
- From:
Chinese Pharmacological Bulletin
2021;37(8):1133-1137
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of miR-125b on HSC proliferation, apoptosis and collagen expression. Methods HSCs were transfected with miR- 125b mimics and inhibitors, and then molecular biology methods were used to detect the expression of Collagen I and HI in HSCs; MIT method was employed to detect HSC proliferation, and flow cytometry to detect HSC apoptotic rate. Results Molecular biological testing found that miR-125b mimics inhibited the expression of Collagen I and HI, and miR-125b inhibitors promoted the expression of Collagen I and HI mRNA; miR-125b mimics inhibited the proliferation of HSC, and miR-125b inhibitors promoted HSC proliferation; miR-125b mimics promoted HSC cell apoptosis, miR- 125b inhibitor inhibited HSC apoptosis, the above experimental results were statistically significant compared with its negative control group ( P < 0. 05 ). Conclusions miR-125b has anti-liver fibrosis effects, which may be related to the regulation of the phenotype of hepatic stellate cells.
