Nifedipine promotes formation of autophagosomes and enhances expression of Beclinl in hepatoma cell line Huh-7
10.3969/j.issn.1001-1978.2022.02.014
- Author:
Cheng ZHOU
1
;
Guang-Liang WANG
2
;
Guang-Yu PAN
3
;
Hong-Tao UAO
4
;
Ning TAN
4
;
Shu-Min YUAN
5
Author Information
1. Graduate School, Cuilin Medical University
2. Faculty of Basic Medical Sciences, Dept of Histology and Embryology, Cuilin Medical University
3. College of Biotechnology, Dept of Biomedical Engineering, Cuilin Medical University
4. Dept of Immunology, Faculty of Basic Medical Sciences, Cuilin Medical University
5. Faculty of Basic Medical Sciences, Dept of Microbiology, Cuilin Medical University
- Publication Type:Journal Article
- Keywords:
autophagosome;
autophagy;
Beclin1;
LC3B-Ⅱ;
liver cancer;
nifedipine
- From:
Chinese Pharmacological Bulletin
2022;38(2):228-233
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of nifedipine on the formation of autophagosomes in hepatoma cell line Huh-7 and its mechanism.Methods Different concentrations of nifedipine were used to interfere with the proliferation of Huh-7 cells in vitro.The effect of nifedipine on the proliferation of Huh-7 cells was detected by cell proliferation experiment and colony formation experiment.The expressions of Beclin1 and LC3B-Ⅱ were detected by Western blot.The effect of nifedipine on the formation of autophagosomes in Huh-7 cells was observed by laser scanning confocal microscopy.Results Nifedipine significantly inhibited the proliferation of Huh-7 cells in a time-and concentration-dependent manner.The IC50 of nifedipine on day 2 was 22.7 mg·L-1.Nifedipine at the concentration of 25 mg·L-1 significantly reduced the colony formation rate of Huh-7 cells compared with the control group, and the inhibition rate of colony formation was(95.46±0.45)%.Western blot analysis showed that nifedipine significantly up-regulated the protein expression levels of Beclin1 and LC3B-Ⅱ.The amount of autophagosomes in nifedipine group cells were more than that of control group, which was observed by laser scanning confocal microscopy.Conclusions Nifedipine significantly inhibits the proliferation of Huh-7 cells and promotes the formation of autophagosomes, which may be related to the up-regulation of Beclin1 protein expression by nifedipine.
