Arbutin isolated from Chinese yam inhibits LPS-induced NRK-52e cell apoptosis by ERp

Bei-Bei ZHANG; Meng-Nan ZENG; Ju-Fang JIA; Peng-Li GUO; Meng LIU; Qin-Qin ZHANG; Wei-Sheng FENG; Xiao-Ke ZHENG

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Arbutin isolated from Chinese yam inhibits LPS-induced NRK-52e cell apoptosis by ERp

Author: Bei-Bei ZHANG ¹ ; Meng-Nan ZENG ¹ ; Ju-Fang JIA ¹ ; Peng-Li GUO ¹ ; Meng LIU ¹ ; Qin-Qin ZHANG ¹ ; Wei-Sheng FENG ¹ ; Xiao-Ke ZHENG ¹
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1. The Engineering and Tech Nology Center for Chinese Medicine Development of Ilenan Province, Henan University of Chinese Medicine
Publication Type:Journal Article
Keywords: arbutin; cell apoptosis; Chinese yam; estrogen; LPS; NRK-52e cell
From: Chinese Pharmacological Bulletin 2022;38(3):403-410
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of arbutin on apoptosis of NRK-52e cells induced by LPS and the potential mechanism.Methods The model of NRK- 52e cells injury was constructed by LPS, and NRK-52e cells were divided into control, LPS ( 1 mg • L 1 ) , low dose arbutin (LPS, 1 mg • L 1 + arbutin, 5 (xmol • L_l ) , high dose arbutin ( LPS, 1 mg • L 1 + arbutin , 10 (xmol • L 1 ) and its corresponding inhibitor THC group ( 1 (xmol • L 1).The cell viability was detected ; the levels of ROS, apoptosis, Ca~' concentration and mitochondrial membrane potential ( MMP ) were detected by flow cytometry; the levels of key apoptosis proteins were detected by in cell western; the binding activity of arbutin with ER(3 was imitated by molecular docking technology, and verified by in cell western.Results Arbutin could effectively regulate the levels of ROS, Ca"+ , apoptosis proteins and ER(3 in NRK-52e cells induced by LPS and inhibit the de- cline of MMP, which is blocked by estrogen receptor inhibitor THC.In addition, arbutin has good binding activity with ERf}.Conclusion This study confirms that arbutin could inhibit LPS-induced apoptosis of NRK-52e cells through ER£.