Study on the regulatory effect of active ingredient group from Periploca forrestii Schltr.Compound on collagin-induced arthritis rats based on gut microbiota
- Author:
Bo WANG
1
;
Hong ZHANG
1
;
Wen-Jing HONG
1
;
Chen-Yang LI
1
;
Jian CHEN
1
;
Lei SHEN
1
;
Qi LI
1
;
Hong ZHANG
2
;
Qi LI
2
Author Information
- Publication Type:Journal Article
- Keywords: ac¬tive ingredient group; collagin-induced arthritis; functional prediction; gut microbiota; high-throughput sequencing; Periploca forrestii Schltr.compound
- From: Chinese Pharmacological Bulletin 2022;38(6):920-928
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the therapeutie effect thritis (CIA) rats based on the variation of gut micro- of active ingredient group ( AIG) in the compounds biota.Methods CIA model rats were induced by in- from Periploca forrestii Schltr.on collagin-induced ar- jection of emulsion containing bovine type II collagen.anrl enzyme-linked immunosorbent assay ( ELISA) was used to investigate the changes of TNF-a and IL-6 in ankle joint of rats in each group.Meanwhile, the gut microbial communities were measured using 16s rRNA high-throughput sequencing technology and its function was predicted by KEGG.Results AIG could signifi¬cantly restrain inflammatory cell infiltration and tissue proliferation in synovial tissues of CIA rats,and allevi¬ate joint swelling while significantly reducing the con¬tents of IL-6 and TNF-a in ankle joint of CIA rats.The analysis of gut microbial communities showed that AIG could increase the relative abundances of Bacte- roidetes, Alloprevotella and Prevotella _9 , and decrease the relative abundances of 1 1 genera such as Dubosiel- la, Lachnospiraceae_NKAA 136_group, etc.in CIA rats.The functional prediction showed that AIG coulrl influ¬ence apoptosis and other signaling pathways.Conclu¬sions AIG can obviously alleviate joint swelling, re¬strain inflammatory cell infiltration and tissue prolifera¬tion in joint synovium of CIA rats,and repair the disor¬dered gut microbiota structure of CIA rats, while affecting its apoptosis pathway and restore the body immunity.
