Recovery of IFN-7-secreting T and NK cells after low dose X-ray radiation injury and the protective effect of Prepared Radix Rehmanniae
- Author:
Shan ZHANG
1
;
Yue ZHANG
1
;
Jia-Xing SHEN
1
;
Li WANG
1
;
Cheng LIU
1
;
Yu-Qi GUO
1
;
Cheng-Fang YAO
1
;
Xiang-Jing CHEN
2
;
Yue WANG
2
;
Ling-Wan KONG
2
Author Information
- Publication Type:Journal Article
- Keywords: CD4+ T cells; CDS + T cells; interferon-y; irradiation; NK cells
- From: Chinese Pharmacological Bulletin 2022;38(6):887-893
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the reeovery of I FN-7- tion and the protective effect of Prepared Radix Reh- secreting T and NK cells after low dose X-ray irradia- manniae ( PRR ).Methods X-ray 2.5Gy was used to establish a mouse irradiated model,and some irradiated mice were used to establish a melanoma lung cancer metastasis model or to be treated by PRR.The propor¬tion and number of Tel , Thl and NK1 cells with the phenotypes of IL-12R and IL-15R were detected by flow cytometry.The transcription levels of IL-12,1L- 15 ,STAT4 and T-bet were detected by RT-qPCR.Re¬sults Within four days after irradiation,Thl ,Tcl and NK1 cells were significantly reduced ( P < 0.05 , P < 0.01).The expression of IL-12R and IL-15R de¬creased in Thl and Tel cells , and increased in NK1 cells ( P < 0.05 ).On day 8 of irradiation , NK1 and Tc 1 cells recovered or exceeded basic level, but Th 1 was still lower than normal level ( P < 0.05 ) , and tumor load of irradiated mice increased significantly (P <0.01).Compared with radiation group, the propor¬tion and absolute numbers of NK1 ,Tel and Thl were up-regulated by PRR (P <0.05 <0.01) ,and tumor load was down-regulated ( P < 0.05 ).Conclusions The impaired reconstitution of Thl cells after irradia¬tion affects the anti-tumor ability.PRR promotes the recover}' of IFN-∗y-secreting T and NK cells after radia¬tion and reduces the risk of tumor metastasis in mice.
