Effects of betulinic acid on epithelial-mesenchymal transition of human gastric cancer MKN-45 cells

Author: Huai-Yu LI ¹ ; Zhao-Xing WAN ¹ ; Hui-Ling DENG ¹ ; Zhen YE ¹ ; Huai-Yu LI ² ; Yun CHEN ³ ; Zi-Yi HU ⁴ ; Jing YE ⁵
Author Information

1. Jiangxi University of Chinese Medicine
2. The First Affiliated Hospital of Guangzhou University of Chinese Medicine
3. Dept of Gastroenterology, First Affiliated Hospital of Gannan Medical University
4. Dept of TCM, The Affiliated Hospital of Jiangxi University of Chinese Medicine
5. School of Clinical Medicine, Jiangxi University of Chinese Medicine
Publication Type:Journal Article
Keywords: betulinic acid; epithelial-mesenchymal transition; gastric cancer; invasion; migration; TGF-β1
From: Chinese Pharmacological Bulletin 2023;39(8):1438-1443
CountryChina
Language:Chinese
Abstract: Aim To observe the effect of betulinic acid (BA) on the migration and invasion of human gastric cancer MKN-45 cells induced by transforming growth factor-pi (TGF-β1), and to explore the effect of BA on epithelial-mesenchymal transition (EMT) and the potential mechanism. Methods The MKN-45 cells were cultivated in vitro, and the effects of different concentrations of BA on the proliferation of MKN-45 cells at 24, 48 and 72 h were detected using CCK-8 method. The effects of BA (5, 10, 20 jjunol • L) and TGF-01 inhibitor LY2109761 (10 ^mol • L"