Effects of Apelin-13/putative receptor protein related to AT1 homodimer on behaviors of human umbilical vein endothelial cells
- Author:
De-Xiu WANG
1
;
Yue YIN
1
;
Hong LU
1
;
Xin CAI
1
;
Jian-She LI
2
;
Chao SONG
2
;
Chang-Hao XIAO
2
;
Tai-Qian WANG
3
;
Xue-Jian WANG
4
Author Information
- Publication Type:Journal Article
- Keywords: Apelin-13; APJ homodimer; HUVECs; migration; proliferation; tube formation
- From: Chinese Pharmacological Bulletin 2023;39(1):51-56
- CountryChina
- Language:Chinese
- Abstract: Aim To explore the effects of putative receptor protein related to ATI (APJ) homodimer on the behaviors-the proliferation, migration and tube formation of human umbilical vein endothelial cells (HU-VECs). Methods HUVECs at logarithmic growth stage were randomly divided into PBS, Apelin-13 + TM1 (APJ monomer group) and Apelin-13 + PBS group (APJ homodimer group). Western blot and Matrix-Assisted Laser Desorption/Ionization Time of Fligh Mass Spectrometry (MALDI-TOF MS) were used to detect the expression of APJ and APJ homodimer in HUVECs, respectively. Real-Time Cell Analyzers (RT-CA) was used to detect the concentration of the maximum effect of Apelin-13. Cell viability was detected by CCK-8. The cell migration ability was detected by scratch test, and the number of tubes formed on matri-gel that made artificial basement membrane was counted. Results Western blot and MALDI-TOF MS showed that APJ and APJ homodimer were expressed in HUVECs. The EC50 of Apelin-13 was 2.26 x 10
