Discussion on safety and related pharmacological mechanism of compound anshen essential oil in relieving sleep disorders

Lei ZHOU; Yue GAO; Lei ZHOU; Wei ZHOU; Yong-Qiang ZHOU; Yue GAO; Li-Yun ZHU; Peng-Yi HU; Ming YANG; Qin ZHENG

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Discussion on safety and related pharmacological mechanism of compound anshen essential oil in relieving sleep disorders

Author: Lei ZHOU ¹ ; Yue GAO ¹ ; Lei ZHOU ² ; Wei ZHOU ² ; Yong-Qiang ZHOU ² ; Yue GAO ² ; Li-Yun ZHU ³ ; Peng-Yi HU ³ ; Ming YANG ³ ; Qin ZHENG ³
Author Information

1. School of Pharmacy Guangdong Pharmaceutical University
2. Institute of Radiation Medicine, Academy ofMilitary Medical Sciences
3. Key Laboratory of Modern Preparation of Traditional Chinese Medicine under Ministry of Education, State Key Laboratory of Innovation Drug and Efficient Energy-Saving Pharmaceutical Equipment, Jiangxi University of Traditional Chinese Medicine
Publication Type:Journal Article
Keywords: compound anshen essential oil; EEG characteristics monitoring; hypnotic effect; pharmacological mechanism; safety evaluation; sleep monitoring technology for micro-motion sensitive mattresses
From: Chinese Pharmacological Bulletin 2023;39(3):580-587
CountryChina
Language:Chinese
Abstract: Aim To study the hypnotic effect and safety of compound anshen essential oil. Methods Gas chromatograph-mass spectrometer (GC-MS) was used to analyze the main active components of compound anshen essential oil. The mouse model of insomnia was established by intraperitoneal injection of para-chloro-phenyl alanine ( PC PA ) , combined with pentobarbital sodium sleep experiment and EEG characteristic monitoring in rats to study the hypnotic effect and mechanism. The safety of compound anshen essential oil was evaluated by acute toxicity test, skin irritation/allergy test and 90-day repeated administration toxicity test. The clinical effect and safety were evaluated by using the sleep monitoring technology for micro-motion sensitive mattress. Results Four components, including Atractylone (34.61%), (+) -Limonene (17.80%) , Linalool (11.63%), and Ocimene (11.67%) , were detected as the main active components of compound anshen essential oil. Compound anshen essential oil in-halation administration for seven days could effectively reduce the autonomic activity of insomnia mice, shorten the sleep latency (P <0.05) , improve the sleep duration, increase of neurotransmitters such as 5-hydroxy tryptamine (5-HT) and -γ-aminobutyric acid (GABA) in brain of mice with insomnia, and the medium dose group had better hypnotic effect. There was no death or adverse reaction in the safety evaluation test. The sleep balance index of 10 subjects with difficulty in falling a-sleep significantly increased (P <0.05), sleep latency was significantly shortened (P <0.05) , total sleep duration and sleep efficiency were improved, and no ad¬verse reactions were found after using the compound anshen essential oil for two days. Conclusions The compound anshen essential oil developed by the research team is safe and effective in relieving sleep disorders, which may be closely related to the co-regulation of the levels of neurotransmitters such as 5-HT and GABA by the four main active components.