Effects of DNMT3A on mouse cardiac fibroblast proliferation and migration under high glucose environment

Author: He SUN ¹ ; Bin TU ¹ ; Kai SONG ¹ ; Yang ZHOU ¹ ; Ji-Ming SHA ¹ ; Sheng-Song XU ¹ ; Hui TAO ¹ ; Rui LI ² ; Ye ZHANG ² ; Hui TAO ² ; Juan WANG ³ ; Feng SUN ³
Author Information

1. Dept of Cardiothoracic Surgery, The Second Hospital of Anhui Medical University
2. Dept of Anesthesiology, The Second Hospital of Anhui Medical University
3. School of Pharmacy, Anhui Medical University
Publication Type:Journal Article
Keywords: cardiac fibroblasts; diabetic cardiomyopathy; DNMT3A; high glucose; migration; proliferation
From: Chinese Pharmacological Bulletin 2023;39(3):555-560
CountryChina
Language:Chinese
Abstract: Aim To investigate the effect of DNA methyltransferase 3A (DNMT3A) on the proliferation and migration of cardiac fibroblasts (CFs) in C57 mice under high glucose environment. Methods The hearts of C57 mice were taken from 1 to 3 days. After cutting and digesting, CFs were extracted by differential adherance centrifugattion and observed under microscope. After cell attachment, the cells were cultured under low glucose (5.5 mmol • L