Effect of Gualou Xiebai Decoction on type II cardiorenal syndrome based on endothelial/epithelial-to-mesenchymal transition and its mechanism
- Author:
Yun-Yun ZHANG
1
;
Xiao-Yu ZHANG
1
;
Hua-Hua WANG
1
;
Yue-Hong SHEN
1
;
Yong-Fang DING
1
;
Ming-Qin SHEN
1
;
Yun-Yun ZHANG
2
;
Xiao-Yu ZHANG
2
;
Hua-Hua WANG
2
;
Yue-Hong SHEN
2
;
Yong-Fang DING
2
;
Ming-Qin SHEN
2
Author Information
- Publication Type:Journal Article
- Keywords: cardiac function; cardiorenal fibrosis; collagen deposition; endothelial/ epithelial-to-mesenchymal transition; Gualou Xiebai Decoction; kidney function; type II cardiorenal syndrome
- From: Chinese Pharmacological Bulletin 2023;39(6):1173-1181
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the protective effects of different doses Gualou Xiebai Decoction (GXD) on type II cardiorenal syndrome (type II CRS) and explore its preliminary mechanisms. Methods The type II cardiorenal syndrome rat model was replicated by li-gating the left anterior descending coronary artery. After 10 weeks of intragastric administration, the cardiac function of the rats in each group was evaluated by echocardiography; serum were collected for biochemical testing; heart and kidney tissue samples were stained with HE and Masson to observe pathological changes. The hydroxyproline content in the heart and kidney was detected. The expression levels of endothelial/epitheli-al-to-mesenchymal transition (EndMT/EMT) related proteins in heart and kidney tissues were detecterd by immunofluorescence double staining ^Western blot. Results The index of heart and kidney organs of the low and high doses of GXD group significantly decreased (P<0. 05, P <0. 01), the cardiac function indexes were significantly improved (P < 0. 05, P < 0. 01). Serum characteristic indexes showed that heart and kidney functions were significantly improved (P < 0. 01). In addition, the pathological changes of the heart and kidney were significantly ameliorated. The hydroxyproline content in heart and kidney correspondingly declined (P < 0. 01). The expression levels of CD31 and E-cadherin were significantly up-regulated (P < 0. 05, P <0. 01), and the expression levels of Vimentin, a-SMA and TGF-f3l were down-regulated in heart and kidney tissues (P < 0. 05, P < 0. 01). Conclusions Therefore, GXD can significantly improve the heart and kidney function of type II CRS rats caused by ligation of the left anterior descending coronary artery, and delay the process of heart and kidney fibrosis. The mechanism may be related to the inhibition of EndMT/ EMT.
