Effect of boschniakia rossica polysaccharides on hypoxia/reoxygenation-induced cardiomyocyte damage by down-regulating miR-302a-3p expression

Author: Hong CHEN ¹ ; Ming-Wei HUANG ¹ ; Zhen-Dong CHENG ¹ ; Hui-Yao LU ¹ ; Chao-Xiang XU ¹ ; Jing-Ying WU ¹ ; Jin-Cheng CHEN ²
Author Information

1. Dept of Cardiology, Second Affiliated Hospital of Fujian Medical University
2. Dept of Pharmacy, Second Affiliated Hospital of Fujian Medical University
Publication Type:Journal Article
Keywords: apoptosis; boschniakia rossica polysaccharides; hypoxia/reoxygenation; miR-302a-3p; oxidative stress; rat cardiomyocyte H9c2
From: Chinese Pharmacological Bulletin 2023;39(7):1241-1247
CountryChina
Language:Chinese
Abstract: Aim To explore the effect of boschniakia rossica polysaccharides ( BRPS ) on cardiomyocyte damage induced by hypoxia/reoxygenation (H/R) and its possible mechanism. Methods H/R was used to induce rat cardiomyocyte H9c2 to establish a cell inju¬ry model, and different doses of BRPS were used to treat H9c2 cells. ELISA method was used to detect the level of MDA and the activity of SOD and GSH-Px. Flow cytometry was used to detect the rate of apopto-sis. qRT-PCR was used to detect the expression of miR-302a-3p. anti-miR-NC and anti-miR-302a-3p were respectively transfected into H9c2 cells and then subjected to H/R treatment. miR-NC and miR-302a-3p mimics were respectively transfected into H9c2 cells and treated with 100 mg • L