Benzyl isothiocyanate induces cell cycle arrest and apoptosis in cervical cancer through activation of p53 and AMPK-FOXO1a signaling pathways
- Author:
Tamasha KURMANJIANG
1
;
Xiao-Jing WANG
1
;
Xin-Yi LI
1
;
Hao WANG
1
;
Guo-Xuan XIE
1
;
Yun-Jie CHEN
1
;
Ting WEN
1
;
Xi-Lu CHENG
1
;
Nuraminai MAIMAITI
1
;
Jin-Yu LI
1
Author Information
- Publication Type:Journal Article
- Keywords: benzyl isothiocyanate; cell apoptosis; cell cycle; cervical cancer; FOXO; p53; transcriptome
- From: Chinese Pharmacological Bulletin 2024;40(1):114-158
- CountryChina
- Language:Chinese
- Abstract: Aim To investigate the effect of benzyl iso-thiocyanate (BITC) on the proliferation of mouse U14 cervical cancer cells and to explore the mechanism of cytotoxicity based on transcriptomic data analysis. Methods The effect of BITC on U14 cell activity was detected by MTT, nuclear morphological changes were observed by Hochest 33258 and fluorescent inverted microscope, cell cycle and apoptosis were determined by flow cytometry, and the transcriptome database of U14 cells before and after BITC (20 μmol · L
