Berberine interferes with RSV infection of HEp-2 cells by inducing mitophagy autophagy

Yu-Juan CUI; Yu-Juan CUI; Hui ZHAO; Dong-Xia SU; Dan-Dong HU

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Berberine interferes with RSV infection of HEp-2 cells by inducing mitophagy autophagy

Author: Yu-Juan CUI ¹ ; Yu-Juan CUI ² ; Hui ZHAO ² ; Dong-Xia SU ² ; Dan-Dong HU ³
Author Information

1. School of Life Science, Northwest Normal University
2. Beijing Vanning Center for DiseasePrevention and Control
3. Beijing Vanning Market SupervisionInspection and Testing Monitoring Center
Publication Type:Journal Article
Keywords: berberine; mitochondria-autophagosome colocalization; mitochondrial membrane potential; mitophagy; NLRP3 inflammasome; ROS; RSV
From: Chinese Pharmacological Bulletin 2024;40(2):308-316
CountryChina
Language:Chinese
Abstract: Aim To explore the effect of berberine (B E) on RSV infected HEp-2 cells and the related mechanism. Methods HEp-2 cells were infected with RSV and treated with BE. Cell viability was assessed using the CCK-8 assay. Protein expression levels of NLRP3, ASC, caspase-1, PINK1, Parkin, Beclinl, p62, LC3 I,LC3 II,and BNIP3 in HEp-2 cells were detected by Western blot. The secretion level of IL-1 p in HEp-2 cells was measured using ELISA. Apoptosis rate and mitochondrial membrane potential of HEp-2 cells were examined by flow cytometry. Mitochondrial ROS (mtROS) in HEp-2 cells was detected through MitoSOX staining. Colocalization of mitochondria and autophagosomes in HEp-2 cells was investigated using immunofluorescence staining. Cyclosporin A was used for validation experiments. Results BE could significantly improve the activity of RSV-infected HEp-2 cells,reduce the apoptosis rate (P < 0. 05), and decrease the activation level of NLRP3 inflammasomes and IL-lp level (P <0. 05); BE improved mitochondrial function by increasing mitochondrial membrane potential and ATP levels,and reduced mtROS. BE significantly promoted the colocalization of mitochondria-autophagosome in RSV infected cells, inducing PINK1/ Parkin and BNIP3 to mediate mitochondrial autophagy; cyclosporine A aggravated RSV infection. Conclusions BE has protective effects on HEp-2 cells infected by RSV. The mechanism may be related to the inhibitory effect of BE on the production of mtROS and the activation of NLRP3 inflammasomes by inducing PINK1/ Parkin and BNIP3-mediated mitochondrial autophagy.