Mechanism and experimental validation of Zukamu granules in treatment of bronchial asthma based on network pharmacology and molecular docking
- Author:
Yan-Min HOU
1
;
Li-Juan ZHANG
1
;
Yu-Yao LI
1
;
Wen-Xin ZHOU
1
;
Hang-Yu WANG
1
;
Jin-Hui WANG
1
;
Ke ZHANG
1
;
Mei XU
2
;
Dong LIU
2
;
Jin-Hui WANG
3
Author Information
- Publication Type:Journal Article
- Keywords: bronchial asthma; molecular docking; network pharmacology; NF - KB / MAPK; ovalbumin; zukamu granules
- From: Chinese Pharmacological Bulletin 2024;40(2):363-371
- CountryChina
- Language:Chinese
- Abstract: Aim To anticipate the mechanism of zuka- mu granules (ZKMG) in the treatment of bronchial asthma, and to confirm the projected outcomes through in vivo tests via using network pharmacology and molecular docking technology. Methods The database was examined for ZKMG targets, active substances, and prospective targets for bronchial asthma. The protein protein interaction network diagram (PPI) and the medication component target network were created using ZKMG and the intersection targets of bronchial asthma. The Kyoto Encyclopedia of Genes and Genomics (KEGG) and gene ontology (GO) were used for enrichment analysis, and network pharmacology findings were used for molecular docking, ovalbumin (OVA) intraperitoneal injection was used to create a bronchial asthma model, and in vivo tests were used to confirm how ZKMG affected bronchial asthma. Results There were 176 key targets for ZKMG's treatment of bronchial asthma, most of which involved biological processes like signal transduction, negative regulation of apoptotic processes, and angiogenesis. ZKMG contained 194 potentially active components, including quercetin, kaempferol, luteolin, and other important components. Via signaling pathways such TNF, vascular endothelial growth factor A (VEGFA), cancer pathway, and MAPK, they had therapeutic effects on bronchial asthma. Conclusion Key components had strong binding activity with appropriate targets, according to molecular docking data. In vivo tests showed that ZKMG could reduce p-p38, p-ERKl/2, and p-I
