Triptolide inhibits proliferation, invasion and migration of human breast cancer MCF-7 cells by regulating miR-142-3p/HSP70 pathway

WANG Jinjun1，2a; CUI Penglai3; CHENG Xin1; QIAN Mengyue2b; ZENG Xiangjun3; XU Zijin3; WANG Yifan3

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Triptolide inhibits proliferation, invasion and migration of human breast cancer MCF-7 cells by regulating miR-142-3p/HSP70 pathway

VernacularTitle:雷公藤内酯醇通过调控miR-142-3p/HSP70通路抑制人乳腺癌MCF-7细胞增殖、侵袭和迁移
Author: WANG Jinjun1，2a ^{1
,

2} ; CUI Penglai3 ^{1
,

2} ; CHENG Xin1 ^{1
,

2} ; QIAN Mengyue2b ^{1
,

2} ; ZENG Xiangjun3 ^{1
,

2} ; XU Zijin3 ^{1
,

2} ; WANG Yifan3 ^{1
,

2}
Author Information

1. Department of Rheumatology, Wuhan Hospital of Traditional Chinese Medicine, Wuhan 430000, Hubei, China; 2a. Graduate School; b. College of Acupuncture, Orthopedics, and Trauma, Hubei University of Traditional Chinese Medicine, Wuhan 430065, Hubei, China;
2. Rehabilitation and Physiotherapy Department, People's Hospital of Wuhan Economic-Technological Development Zone [Hannan District], Wuhan 430090, Hubei, China
Publication Type:Journal Article
Keywords: 乳腺癌；雷公藤内酯醇；MCF-70细胞；增殖；侵袭；迁移；miR-142-3p/HSP70信号通路
From: Chinese Journal of Cancer Biotherapy 2024;31(3):240-246
CountryChina
Language:Chinese
Abstract: [摘要] 目的：探究雷公藤内酯醇（TP）通过miR-142-3p/HSP70信号通路对人乳腺癌MCF-7细胞恶性生物学行为的影响。方法：常规培养MCF-7细胞，将其分为6组：对照组、TP组、miR-142-3p inhibitor组、TP+inhibitor组、miR-142-3p mimic组和TP+mimic组，用转染试剂将相应的核酸或质粒转染MCF-7细胞。qPCR法、EdU细胞增殖实验、Transwell小室实验、细胞划痕实验、WB法分别检测转染后各组MCF-7细胞中miR-142-3p和HSP70 mRNA的表达，MCF-7细胞的增殖、侵袭、迁移能力和HSP70蛋白表达水平。结果：TP或miR-142-3p过表达能显著促进MCF-7细胞中miR-142-3p和HSP70的表达，敲减miR-142-3p则可明显抑制MCF-7细胞中miR-142-3p和HSP70的表达，TP可逆转由敲减miR-142-3p对MCF-7细胞中miR-142-3p和HSP70表达的影响；TP、过表达miR-142-3p均可明显抑制MCF-7细胞的增殖、迁移和侵袭能力（均P<0.05），敲减miR-142-3p则均可促进MCF-7细胞的增殖、迁移和侵袭能力（均P<0.05），TP可逆转由敲减miR-142-3p对MCF-7细胞恶性生物学行为的影响（均P<0.05）。结论：TP可通过调控miR-142-3p/HSP70信号通路，进而抑制MCF-7细胞的增殖、侵袭和迁移能力。
Full text:20240305.pdf