Guiqi Yiyuan Ointment protects rat left lung from bystander effect of right lung injury induced by ~(12)C~(6+) beam.
10.19540/j.cnki.cjcmm.20230914.702
- Author:
Tian-Xing MA
1
;
Jin-Tian LI
2
;
Juan LI
3
;
Yi ZHANG
1
;
Jian-Qing LIANG
4
Author Information
1. Clinical College of Chinese Medicine, Gansu University of Chinese Medicine Lanzhou 730000, China.
2. Clinical College of Chinese Medicine, Gansu University of Chinese Medicine Lanzhou 730000, China Dunhuang Key Laboratory of Medicine and Transformation, Ministry of Education Lanzhou 730000, China.
3. School of Basic Medicine, Gansu University of Chinese Medicine Lanzhou 730000, China.
4. Dunhuang Key Laboratory of Medicine and Transformation, Ministry of Education Lanzhou 730000, China School of Basic Medicine, Gansu University of Chinese Medicine Lanzhou 730000, China.
- Publication Type:Journal Article
- Keywords:
bystander effect of radiation;
heavy ions;
inflammasome;
reactive oxygen species(ROS)
- MeSH:
Rats;
Animals;
NLR Family, Pyrin Domain-Containing 3 Protein/metabolism*;
NF-kappa B/metabolism*;
Inflammasomes/metabolism*;
Lung Injury/genetics*;
Reactive Oxygen Species/metabolism*;
Bystander Effect;
Ointments;
Rats, Wistar;
Lung/metabolism*;
Caspase 1/metabolism*;
RNA, Messenger;
Superoxide Dismutase
- From:
China Journal of Chinese Materia Medica
2023;48(24):6740-6748
- CountryChina
- Language:Chinese
-
Abstract:
This study observed the effects of Guiqi Yiyuan Ointment(GQYY) on the left lung subjecting to bystander effect of right lung injury induced by ~(12)C~(6+) beam in rats and decipher the underlying mechanism from NOD-like receptor protein 3(NLRP3)/apoptosis-associated speck-like protein containing a CARD(ASC)/cysteinyl aspartate specific proteinase-1(caspase-1) pathway. Wistar rats were randomized into 7 groups: blank, model, inhibitor [200 mg·kg~(-1), N-acetylcysteine(NAC)], western drug [140 mg·kg~(-1) amifostine(AMI)], and high-, medium-, and low-dose(4.8, 2.4, and 1.2 g·kg~(-1), respectively) GQYY groups. The model of bystander effect damage was established by 4 Gy ~(12)C~(6+) beam irradiation of the right lung(with the other part shielded by a lead plate). The pathological changes in the lung tissue, the level of reactive oxygen species(ROS) in the lung tissue, and the levels of superoxide dismutase(SOD) and malondialdehyde(MDA) in the serum were observed and measured in each group. Furthermore, the mRNA and protein levels of NLRP3, ASC, caspase-1, and phosphorylated nuclear factor-κB p65(p-NF-κB p65)/nuclear factor-κB p65(NF-κB p65) were determined. Compared with the blank group, the model group showed thickened alveolar wall, narrowed alveolar cavity, and presence of massive red blood cells and inflammatory infiltration in the alveolar wall and alveolar cavity. In addition, the model group showed elevated ROS levels in both left and right lungs, elevated MDA level, lowered SOD level, and up-regulated mRNA and protein levels of NLRP3, ASC, caspase-1, and p-NF-κB p65/NF-κB p65. Compared with the model group, the drug administration in all the groups reduced inflammatory cell infiltration in the lung tissue. The inhibitor group and the western drug group showed enlarged alveolar cavity, thinned interstitium, and reduced inflammation. There was a small amount of alveolar wall rupture in the high-and medium-dose GQYY groups and reduced inflammatory cell infiltration in the low dose GQYY group. Compared with the model group, drug administration lowered level of ROS in the left and right lungs, lowered the MDA level, elevated the SOD level, and down-regulated the mRNA and protein levels of NLRP3, ASC, caspase-1, and p-NF-κB p65/NF-κB p65. GQYY can effectively reduce the damage caused by radiation and bystander effect, which may be associated with the ROS-mediated NLRP3 inflammasome activation.
