Component profile analysis of Sanhan Huashi Formula based on UHPLC-LTQ-Orbitrap-MS, GC-MS, and UHPLC-QQQ-MS/MS.
10.19540/j.cnki.cjcmm.20230614.303
- Author:
Meng-Xiao WANG
1
;
Chen-Si YAO
2
;
Hai-Yu ZHAO
1
;
Wei-Hao WANG
1
;
Yan ZHANG
1
;
Ke-Ke LUO
1
;
Nan SI
1
;
Bao-Lin BIAN
1
;
Tao LI
3
;
Xiao-Lu WEI
1
;
Meng-Yao TIAN
1
;
Yan-Yan ZHOU
1
;
Hong-Jie WANG
1
;
Min LI
2
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
2. Guang'anmen Hospital, China Academy of Chinese Medical Sciences Beijing 100053, China.
3. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China Beijing Key Laboratory of Traditional Chinese Medicine Basic Research on Prevention and Treatment of Major Diseases, Experimental Research Center, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
GC-MS;
Sanhan Huashi Formula;
UHPLC-LTQ-Orbitrap-MS;
UHPLC-QQQ-MS/MS;
active components;
content determination
- MeSH:
Tandem Mass Spectrometry;
Chromatography, High Pressure Liquid;
Gas Chromatography-Mass Spectrometry;
Reproducibility of Results;
Drugs, Chinese Herbal/chemistry*
- From:
China Journal of Chinese Materia Medica
2023;48(22):6066-6074
- CountryChina
- Language:Chinese
-
Abstract:
This study comprehensively analyzed the active components of Sanhan Huashi Formula using qualitative and quantitative mass spectrometry techniques, laying the foundation for understanding its pharmacological substance basis. UHPLC-LTQ-Orbitrap-MS and GC-MS technologies were used to analyze and identify the volatile and non-volatile components in Sanhan Huashi Formula. UHPLC-QQQ-MS/MS technology was used to simultaneously determine the content of 27 major active components in the formula. The results showed that 308 major chemical components were identified in Sanhan Huashi Formula, among which 60 compounds were identified by comparing with reference standards, mainly including alkaloids, flavonoids, coumarins, triterpenoid saponins, amino acids, and nucleosides. GC-MS technology preliminarily identified 52 volatile compounds, with γ-eudesmol and β-eudesmol as the main components. The quantitative results demonstrated good linearity(r>0.99) for the 27 active components, indicating the stability, simplicity, and reliability of the established method. Among them, amygdalin, nodakenin, arecoline, ephedrine, and pseudoephedrine had relatively high content and were presumably the main pharmacologically active substances. In conclusion, this study systematically and comprehensively characterized the major chemical components and patterns in Sanhan Huashi Formula, providing a basis for understanding its pharmacological mechanisms and clinical applications.
