UPLC-MS/MS determination of active components from Wuzhuyu Decoction in plasma and brain tissues of nitroglycerin-induced migraine rats.
10.19540/j.cnki.cjcmm.20190624.502
- Author:
Yong-Song XU
1
;
Feng QIU
1
;
Sha WU
1
;
Rui HE
1
;
Mu-Xin GONG
1
;
Zhi-Min WANG
2
Author Information
1. School of Traditional Chinese Medicine, Capital Medical University Beijing 100069, China Beijing Key Laboratory of Traditional Chinese Medicine Collateral Disease Theory Research Beijing 100069, China.
2. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences Beijing 100700, China.
- Publication Type:Journal Article
- Keywords:
UPLC-MS/MS;
evodiamine;
ginsendside Rb_1;
limonin;
pharmacokinetic;
rutaecarpine;
rutaevine
- MeSH:
Administration, Oral;
Animals;
Brain/drug effects*;
Brain Chemistry;
Chromatography, High Pressure Liquid;
Drugs, Chinese Herbal/therapeutic use*;
Male;
Migraine Disorders/drug therapy*;
Nitroglycerin;
Plasma/chemistry*;
Rats;
Rats, Sprague-Dawley;
Reproducibility of Results;
Tandem Mass Spectrometry
- From:
China Journal of Chinese Materia Medica
2020;45(3):645-654
- CountryChina
- Language:Chinese
-
Abstract:
A sensitive and specific ultra-performance liquid chromatography-mass spectrometry(UPLC-MS/MS) method was deve-loped for analysis of rutaecarpine(Ru), evodiamine(Ev), rutaevine(Rv), limonin(Li), ginsendside Rb_1(Rb_1), ginsendside Re(Re) in rat plasma and brain tissues of nitroglycerin-induced migraine rats. Male healthy Sprague-Dawley(SD) rats were orally given multiple dose of optimized(OS) and un-optimized Wuzhuyu Decoction(UNOS), and their blood samples and brainstem were collected at different time points after injection of nitroglycerin(10 mg·kg~(-1)) into the frontal region. The drug concentrations of the 6 analytes in plasma and brainstem were determined by UPLC-MS/MS method. Subsequently, the main pharmacokinetics parameters of plasma were calculated by using Phoenix WinNolin 5.2.1 software. The methodological test showed that all of analytes in both plasma and brainstem homogenate exhibited a good linearity within the concentration range(r>0.994 7). The intra-day and inter-day accuracy, precision, matrix effect, stability of the investigated components meet the requirements for biopharmaceutical analysis. The developed method was successfully applied in pharmacokinetic studies on abovementioned ingredients in rat plasma and brain stem. The plasma pharmacokinetic parameters of active ingredients in two different Wuzhuyu Decoction group were compared, it was found that Rb_1 had higher t_(1/2), T_(max), C_(max), AUC_(0-24 h) and AUC_(0-∞ )in OS group. Meanwhile, Ev had higher t_(1/2) and T_(max) but lower C_(max), AUC_(0-24 h) and AUC_(0-∞), Ru has higher t_(1/2 )but lower C_(max), AUC_(0-24 h) and AUC_(0-∞ )in OS group. The brain tissue distribution of each component were compared between the two groups, the component with higher content in OS, such as Ru at 30 min and 2 h after administration, Ev at 30 min, Rb_1 at 30 min and Rb_1 at 2 h after administration have lower brain tissue distribution than those in UNOS group, while the component with higher content in UNOS, such as Rv at 30 min, 2 h and 12 h after administration had higher brain tissue distribution than those in OS group.
