Regulatory effect of Di'ao Xinxuekang on TLR4/MyD88/NF-κB signaling pathway in atherosclerotic rats.
10.19540/j.cnki.cjcmm.20190827.402
- Author:
Wei-Zhi ZHANG
1
;
Guo-Ying LI
1
;
Qin QI
1
;
Sha NA
1
;
Lei LYU
1
;
Guang-Liang CHEN
1
Author Information
1. School of Integrated Traditional and Western Medicine, Anhui University of Chinese Medicine Hefei 230038, China Key Laboratory of Chinese Compound Formula Research of Anhui Province Hefei 230038, China.
- Publication Type:Journal Article
- Keywords:
Di′ao Xinxuekang;
TLR4/MyD88/NF-κB signaling pathway;
atherosclerosis;
prevention and treatment
- MeSH:
Animals;
Aorta/pathology*;
Atherosclerosis/drug therapy*;
Atorvastatin;
Drugs, Chinese Herbal/pharmacology*;
Interleukin-6/blood*;
Interleukin-8/blood*;
Lipids/blood*;
Myeloid Differentiation Factor 88/metabolism*;
Random Allocation;
Rats;
Rats, Sprague-Dawley;
Signal Transduction;
Toll-Like Receptor 4/metabolism*;
Transcription Factor RelA/metabolism*;
Tumor Necrosis Factor-alpha/blood*
- From:
China Journal of Chinese Materia Medica
2020;45(3):602-608
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this paper was to observe the effect of Di'ao Xinxuekang(DXXK) on TLR4/MyD88/NF-κB signaling pathway in atherosclerotic rats, and to explore its anti-atherosclerotic mechanism. Sixty SD rats were randomly divided into normal group, model group, atorvastatin group(4.0 mg·kg~(-1)), and DXXK groups(100, 30, 10 mg·kg~(-1)), with 10 rats in each group. The atherosclerosis model was induced by high fat diet plus vitamin D_2. Experimental drugs were administered intragastrically once daily for 8 weeks starting from the 9 th week. Biochemical analyzers were used to detect levels of triglyceride(TG), total cholesterol(TC), low-density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C) in blood lipid. The levels of serum tumor necrosis factor(TNF)-α, interleukin(IL)-6 and IL-1β were detected by ELISA. Pathological changes of aortic tissues were observed by using Sudan Ⅳ and HE staining. The mRNA and protein expressions of TLR4, MyD88 and NF-κB p65 in aortic tissues were detected by RT-PCR and Western blot, respectively. As compared with the model group, TC, TG, and LDL-C levels in serum were significantly decreased, HDL-C content was significantly increased, and levels of TNF-α, IL-6, and IL-1β in serum were significantly decreased in atorvastatin group and DXXK high and middle dose groups. Aortic lesions in atorvastatin group and DXXK group were significantly improved, and the mRNA and protein expressions of TLR4, MyD88, NF-κB p65 in the aorta were decreased. DXXK has a preventive and therapeutic effect on atherosclerosis in rats, and its mechanism may be related to inhibiting inflammatory reaction by regulating TLR4/MyD88/NF-κB signal transduction, thereby inhibiting the progression of atherosclerosis.
