Chemome profiling of Citri Reticulatae Pericarpium using UHPLC-IT-TOF-MS.
10.19540/j.cnki.cjcmm.20191106.201
- Author:
Ke ZHANG
1
;
Xia XU
1
;
Ting LI
1
;
Yue-Lin SONG
2
;
Jun LI
2
;
Yun-Fang ZHAO
1
;
Qing-Qing SONG
1
;
Peng-Fei TU
1
Author Information
1. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China.
2. Modern Research Center for Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine Beijing 100029, China Beijing Key Lab for Quality Evaluation of Chinese Meteria Medica, Beijing University of Chinese Medicine Beijing 100029, China.
- Publication Type:Journal Article
- Keywords:
3-hydroxy-3-methylglutarate substitution;
Citri Reticulatae Pericarpium;
chemome profiling;
mass fragmentation patterns;
polymethoxyflavones
- MeSH:
Chromatography, High Pressure Liquid;
Citrus/chemistry*;
Drugs, Chinese Herbal/chemistry*;
Mass Spectrometry;
Medicine, Chinese Traditional;
Phytochemicals/analysis*
- From:
China Journal of Chinese Materia Medica
2020;45(4):899-909
- CountryChina
- Language:Chinese
-
Abstract:
Citri Reticulatae Pericarpium(CRP, Chinese name: Chenpi) is one of the most famous edible traditional Chinese medicines(TCMs). CRP was first recorded as top grade TCM in Shennong Bencao Jing attributing to the benefits such as regulating Qi, tonifying spleen, eliminating dampness and eliminating phlegm, and has been widely utilized for the treatments of abdominal fullness and distention, vomiting and diarrhea, as well as phlegm cough. CRP is also widely popular as spice in food industry. Because of the wide cultivation, a number of brands that exhibit extensive price range can be found in the market, resulting in a great challenge for grading. Herein, an attempt was made to in-depth chemome profiling for the sake of providing meaningful information of the universal quality control of CRP. A new core-shell column packed with adamantylethyl substituted silica gel particles was deployed for chromatographic separations and IT-TOF-MS that is advantageous at providing abundant high resolution molecular and fragment ions was employed for qualitative detection. A total of 62 components were observed and 61 ones were structurally annotated according to proposing mass fragmentation patterns, matching with reference compounds and relevant databases, and the chemical families included flavone, limonin, etc. In particular, ten compounds bearing 3-hydroxy-3-methylglutarate substitute were detected from CRP for the first time. Above all, the chemical profile of CRP was characterized and the findings are meaningful for the in-depth quality assessment and efficacy material clarification of CRP.
