DNA fingerprinting identification of bile power(bile)medicines.
10.19540/j.cnki.cjcmm.20200105.106
- Author:
Na TIAN
1
;
Yuan YUAN
2
;
Yan JIN
2
;
Quan YANG
3
;
Tian ZHANG
2
;
Jun-de LI
2
;
Li WANG
2
;
Chao JIANG
2
;
Lu-Qi HUANG
1
Author Information
1. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
2. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences Beijing 100700, China.
3. College of Traditional Chinese Medicine, Guangdong Pharmaceutical University Guangzhou 510006, China.
- Publication Type:Journal Article
- Keywords:
DNA fingerprinting;
STR typing;
adulteration;
bile powder;
molecular identification;
traditional animal medicine
- MeSH:
Animals;
Bile/chemistry*;
Cattle;
Chickens;
DNA Fingerprinting;
Materia Medica/analysis*;
Medicine, Chinese Traditional;
Sheep;
Swine;
Ursidae
- From:
China Journal of Chinese Materia Medica
2020;45(5):1064-1069
- CountryChina
- Language:Chinese
-
Abstract:
The pig bile powder, bovine bile powder, snake bile, sheep bile, goose bile powder, and bear bile powder were contained by the Chinese Pharmacopoeia. The bile power medicine has a long history in traditional Chinese medicine and definite effect. However, the medicine of bile powder(bile) are similar in morphology. Besides, many medicine lack specific microscopic identification characteristics and chemical characteristics. There is a risk of adulteration, especially when the fake medicine were mixed in authentic medicine, it is difficult to detection. The key to control the quality and ensures the clinical efficacy is the good or bad, true or false of the bile power medicine. The STR typing technology is a method that according to differential typing of PCR amplified lengths to compare and identify individual organisms. Based on the principle of STR typing, the easily, rapid DNA fingerprinting method to identify the bile power and adulteration was established.The original animal or bile powder of pigs, cattle, sheep, chickens, ducks, geese, snakes, bears, fish were collected, the 12 S-L1091/12 S-H1478 and 16 S-L3428/16 S-H3667 was obtained by sifted, the DNA fingerprinting of the bile power and adulteration was obtained by STR typing. Every species has different STR fingerprints, so different species can be identified. Besides, the fingerprints have both the authentic and fake's information, the adulteration of authentic and fake can be identified. Therefore, the method to identify the bile power and adulteration was achieved through the combination of two primers. The DNA fingerprinting method established in this study can also be used for other animal medicine.
