Expression of GAB1 in ER-positive breast cancer and its effect on MCF-7 cells’ metastasis
- VernacularTitle:GAB1在ER阳性乳腺癌组织中的表达及其对MCF-7细胞转移能力的影响
- Author:
Shimin ZHANG
1
;
Hongmei DONG
2
;
Ruijun ZHAO
3
Author Information
- Publication Type:Journal Article
- Keywords: ER-positive breast cancer; GAB1; metastasis; prognosis
- From: Journal of Xi'an Jiaotong University(Medical Sciences) 2021;42(4):574-579
- CountryChina
- Language:Chinese
- Abstract: 【Objective】 To evaluate the expression of GAB1 in ER-positive breast cancer and its effect on MCF-7 cells’ metastasis. 【Methods】 The expression of GAB1 in ER-positive breast cancer tissues was detected by immunohistochemistry. We analyzed the relationship of GAB1 expression with the patients’ clinicopathological features and prognosis. The invasion and metastasis abilities of MCF-7 cells after silencing GAB1 expression were observed by Transwell assay. The effect of GAB1 expression on the EMT of breast cancer cells was analyzed by Western blotting. 【Results】 Immunohistochemical staining showed that GAB1 expression had significant correlation with lymph node metastasis(P=0.014) and stage(P=0.011). Transwell results showed that shGAB1 significantly inhibited the migration and invasion of human breast cancer MCF-7 cells. Western blotting results showed that shGAB1 significantly increased E-cadherin expression and decreased Vimentin expression. Kaplan-Meier analysis revealed that ER-positive breast cancer patients with high GAB1 expression tumors had a significantly worse relapse-free survival rate than those with low GAB1 expression tumors(P<0.001). Multivariate analysis showed that stage and GAB1 expression were independent predictors of survival. 【Conclusion】 GAB1 is highly expressed in ER-positive breast cancer. ShGAB1 expression can inhibit the migration, invasion and EMT of breast cancer cells. GAB1 might be used as one of the valuable markers for prognosis in patients with ER-positive breast cancer.
