Exploring the mechanism of Xihuang Pill's anti-hyperplasia of mammary glands effect based on UPLC-Q-orbitrap-MS technology
10.16438/j.0513-4870.2023-0417
- VernacularTitle:基于UPLC-Q-orbitrap-MS技术探究西黄丸抗乳腺增生的作用机制
- Author:
Jing-rui WANG
1
;
Rui TAO
1
;
Xue-li MA
1
;
Jun-liang WANG
1
;
Tao HAN
2
Author Information
1. School of pharmacy, Gansu University of Traditional Chinese Medicine (TCM), Lanzhou 730000, China
2. School of pharmacy, Gansu University of Traditional Chinese Medicine (TCM), Lanzhou 730000, China; Key Laboratory of Pharmacology and Toxicology of TCM of Gansu Province, Lanzhou 730000, China
- Publication Type:Research Article
- Keywords:
hyperplasia of mammary glands;
UPLC-Q-orbitrap-MS;
AGE-RAGE signaling pathway;
Xihuang Pill
- From:
Acta Pharmaceutica Sinica
2024;59(1):214-224
- CountryChina
- Language:Chinese
-
Abstract:
Based on UPLC-Q-orbitrap-MS and biological network analysis tools, the mechanism of Xihuang Pill in improving hyperplasia of mammary glands was systematically analyzed. The rat model of hyperplasia of mammary glands was established by intramuscular injection of estradiol benzoate and progesterone. LC-MS tissue metabolomics was used to explore the key metabolites and metabolic pathways of Xihuang Pill in improving hyperplasia of mammary glands in rat. The network analysis of the key metabolites regulated by Xihuang Pill was carried out by integrating biological network analysis tools, focusing on the key metabolic pathways, and exploring the potential targets of Xihuang Pill to improve hyperplasia of mammary glands. Compared with the control group, there were significant differences in the content of 49 differential metabolites in the tissues of the model group (P < 0.05). Xihuang Pills could significantly call back 17 metabolites such as L-alanine, threonine, indole-3-carboxylic aldehyde, lysine, arginine, alanylleucine, glycyltyrosine, γ-glutamyl leucine, vitamin B3, serine leucine, threonine leucine, isoleucine glutamic acid, γ-glutamyl tyrosine, decanoyl-L-carnitine, uric acid, leucylleucine, S-adenosyl-methionine. Further network analysis and literature research on the key metabolites regulated by Xihuang Pills showed that the AGE-RAGE signaling pathway may be one of the important pathways for Xihuang Pills to improve hyperplasia of mammary glands. STAT3, MAPK1, EGFR, CASP3, CASP8, PRKCA and JUN in the AGE-RAGE signaling pathway may be potential targets for Xihuang Pills to improve hyperplasia of mammary glands. The animal experiment operations involved in this paper follow the provisions of the Animal Ethics Committee of Gansu University of Traditional Chinese Medicine and pass the ethical review of animal experiments (approval number: 2022-705).
