Identification of Original Plants of Lycii Cortex by Multiplex Allele-specific PCR
10.13422/j.cnki.syfjx.20230816
- VernacularTitle:地骨皮的多重位点特异性PCR鉴别
- Author:
Yang SHI
1
;
Li HU
1
;
Yuyang ZHAO
1
;
Chao JIANG
1
;
Yan JIN
1
;
Jing MU
1
;
Yuan YUAN
1
Author Information
1. National Resource Center for Chinese Materia Medica， China Academy of Chinese Medical Sciences， Beijing 100700， China
- Publication Type:Journal Article
- Keywords:
Lycii Cortex;
Lycium chinense;
Lycium barbarum;
molecular identification;
polymerase chain reaction
- From:
Chinese Journal of Experimental Traditional Medical Formulae
2024;30(4):35-41
- CountryChina
- Language:Chinese
-
Abstract:
ObjectiveTo identify Lycium chinense and L. barbarum as the original plants of Lycii Cortex simply and efficiently by multiple allele-specific polymerase chain reaction （PCR）. MethodThe chloroplast genome sequences of L. chinense and L. barbarum were downloaded from the Chloroplast Genome Information Resource （CGIR）， and then IdenDSS was employed to screen out the specific single nucleotide polymorphism （SNP） sites between the two plants. Primer 5.0 was used to design the specific primers， including primers GQ-F/R for identifying L. chinense and primers NX-F/R for identifying L. barbarum. Furthermore， the primer concentration ratio， annealing temperature， cycles， and Taq enzyme were optimized to establish the optimal PCR system and conditions for plant identification. Finally， the applicability of the established method was examined with the plant samples collected from different regions. ResultThe PCR with GQ-F/R∶NX-F/R concentration ratio of 2∶1 at the annealing temperature at 59 ℃ and for 30 cycles showed specific bands at 183 bp and 295 bp， respectively， for L. chinense and L. barbarum samples from different regions. ConclusionThe established PCR approach can simply， rapidly， and efficiently identify the original plants of Lycii Cortex， serving as a new method for the discrimination between L. chinense and L. barbarum. Moreover， the method provides technical support for the research and development of classic famous prescriptions containing Lycii Cortex.
