Frequency analysis of RHD*1227A allele among Rh negative Han population and random population in Liaoning
10.13303/j.cjbt.issn.1004-549x.2021.05.011
- VernacularTitle:RHD*1227A等位基因在辽宁汉族Rh阴性人群和随机人群中的频率分析
- Author:
Xu ZHANG
1
;
Xiaofeng LI
1
;
Jianping LI
1
Author Information
1. Shenyang Central Blood Station (Liaoning Blood Center), Shenyang 110044, China
- Publication Type:Journal Article
- Keywords:
RHD gene;
Del phenotype;
RHD*1227A allele;
allele frequency
- From:
Chinese Journal of Blood Transfusion
2021;34(5):486-488
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the frequency of RHD*1227A allele in Rh negative Han polulation and random population in Liaoning. 【Methods】 Del phenotype was screened by absorption-elution test, the RHD*1227A allele was screened by PCR-SSP and confirmed by nucleotide sequence analysis of RHD full coding region, and the heterozygosity of RHD gene was detected by hybridization technique. 【Results】 24 case of Del phenotype were detected by the absorption-elution test among 117 Rh negative individuals. 23 RHD*1227A allele carriers were detected by PCR-SSP and sequencing-based typing (SBT). Genotype of 19 individuals was RHD*1227A/RHD*01N.01, while the other 4 was homozygous RHD*1227A/ RHD*1227A.11 individuals were detected as RHD*1227A allele among 1 045 random blood donors, among which 9 were RHD*1227A/RHD*01 and 2 were RHD*1227A/RHD*01N.01. 【Conclusion】 The frequency of RHD*1227A allele is 0.115 4 in Rh negative Han population and 0.005 3 in random population in Liaoning..
