Effect of trehalose on platelets stored at 4℃ in vitro
10.13303/j.cjbt.issn.1004-549x.2022.03.001
- VernacularTitle:海藻糖对4℃体外保存血小板的影响
- Author:
Wenjuan LI
1
;
Yujie KONG
2
;
Li TIAN
2
;
Zhong LIU
3
Author Information
1. School of Public Health, Anhui Medical University, Hefei 230032, China
2. Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College
3. School of Public Health, Anhui Medical University, Hefei 230032, China; Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College
- Publication Type:Journal Article
- Keywords:
M-Sol;
trehalose;
CD62p positive rate;
platelet storage lesion
- From:
Chinese Journal of Blood Transfusion
2022;35(3):237-241
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To investigate the effect of trehalose added to M-Sol on platelet preservation at 4℃ in vitro. 【Methods】 Seven bags of ABO homotype concentrated platelets were randomly selected and divided into 7 groups according to storage temperature, preservation medium and trehalose concentration: 22℃+ PRP group, PRP group, M-Sol+ PRP group, M-Sol+ PRP+ 1 g/L trehalose group, M-Sol+ PRP+ 5 g/L trehalose group, M-Sol+ PRP+ 15 g/L trehalose group and M-Sol+ PRP+ 25 g/L trehalose group. PRP group and M-Sol preservation groups were stored at 4℃. Plt, PDW, MPV, maximum aggregation, CD62p positive rate, GLU and LAC concentration were detected on the 1st, 3rd, 5th and 7th day after preservation, and the changes of GLU and LAC concentration within 7 days were calculated. 【Results】 With the extension of preservation time, Plt decreased in all groups, and there was no significant difference among groups at the same time (P>0.05). PDW and MPV increased in all groups. When preserved to the 7th day, the PDW 10.22±0.43(fL) and MPV 8.74±0.40(fL) of M-Sol+ PRP+ 5 g/L trehalose group were the lowest, which was significantly different from that of 22℃+ PRP group (P<0.05). The changes of GLU and LAC concentration within 7 days were the lowest in M-Sol+ PRP+ 5g/L trehalose group, and the change of GLU concentration within 7 days was significantly different from that of 22℃+ PRP group (P<0.05), but not significantly different from that of PRP group (P>0.05). During the preservation period, the maximum aggregation degree of each group decreased gradually. Except for the PRP group, the maximum aggregation degree of the M-Sol+ PRP+ 5g/L trehalose group was the highest(27.29±6.62), which was significantly higher than that of the 22℃+ PRP group on the 7th day after preservation (P<0.05). On the 7th day after preservation, the positive rate of CD62p in M-Sol+ PRP+ 5g/L trehalose group was the lowest(15.46±2.46), and there was significantly different compared with other groups (P<0.05). 【Conclusion】 Adding appropriate concentration of trehalose to M-Sol can inhibit platelet activation at 4℃ and reduce platelet storage lesion.
