Frequency of JK (a-b-) in Yichang: genetic pedigree analysis of Jk (a-b-) and Jka weak expression
10.13303/j.cjbt.issn.1004-549x.2022.02.006
- VernacularTitle:宜昌地区献血者Jk(a－b－)频率与Jka弱表达表型分子遗传机制研究
- Author:
Juncui GAO
1
;
Yurong YUAN
1
;
Yuxiang LIN
2
;
Haojun ZHANG
3
;
Shangen ZHENG
4
Author Information
1. Yichang Red Cross Central Blood Station, Yichang 443005, China
2. Medical School and the First Affiliated Hospital of Xi′an Jiaotong University; Baorui Biotechnology Taizhou Co. Ltd.
3. Baorui Biotechnology Taizhou Co. Ltd.
4. Central Theater General Hospital
- Publication Type:Journal Article
- Keywords:
Jk(a-b-);
Jkaw+;
pedigree analysis;
Kidd blood group;
SLC14A1 gene
- From:
Chinese Journal of Blood Transfusion
2022;35(2):154-156
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the molecular hereditary and frequency of Jk(a-b-) in blood donors in Yichang. 【Methods】 A total of 49 999 samples from Yichang Red Cross Central Blood Station were screened for Jk(a-b-) by urea hemolysis test(2 mol /L). The phenotypes of JK (a-b -) probands and their families were confirmed by monoclonal anti-Jka and anti-Jkb, and the whole exon of SLC14A1 gene was sequenced. 【Results】 The frequency of Jk(a-b-) in Yichang blood donors was 0.004% (2/49 999), and the exon sequencing of SLC14A1 gene confirmed that both two probands were JK*02N.01 caused by c. 342-1G>A homozygous mutation.Besides, JK*01W.01 allele was observed in the pedigree analysis, and weak expression of Jka was found in 4 out of 11 family members. 【Conclusion】 The frequency of JK (a-b -) in Yichang blood donors is similar to those in Shanghai 0.004%(2/48 400), and both caused by JK * 02N.01 allele with high frequency in Southeast Asia. The epidemiological survey of JK * 01w.01 allele frequency should be further performed.
