Analysis of HLA allele-specific antibodies and Eplets in patients with platelet transfusion refractoriness
10.13303/j.cjbt.issn.1004-549x.2022.09.006
- VernacularTitle:血小板输注无效患者HLA等位基因特异性抗体及其表位的分析
- Author:
Bing ZHANG
1
;
Xinyu HUANG
1
;
Ying LIU
1
;
Gang XU
1
;
Shu CHEN
1
;
Xianguo XU
1
;
Faming ZHU
1
Author Information
1. Blood Center of Zhejiang Province, Key Laboratory of Blood Safety Research of ZhejiangProvince, Hangzhou 310052, China
- Publication Type:Journal Article
- Keywords:
platelet transfusion refractoriness;
HLA allele-specific antibodies;
HLA-Ⅰ antibodies;
Structural Eplet
- From:
Chinese Journal of Blood Transfusion
2022;35(9):907-910
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To analyze the specificity and Eplets of HLA allele-specific antibody in patients with platelet transfusion refractoriness (PTR). 【Methods】 HLA-A and B genotypes were detected by PCR-SBT, and HLA-Ⅰ antibodies in patients′ serum were detected by Luminex single antigen beads coating method. IPD-IMGT/HLA Database was used to find the differential amino acids of allele-specific antibodies, and HLA Eplet database was used to analyze the registry Eplets. 【Results】 HLA allele-specific antibodies were found in 12 out of 82 patients with PTR.After sequence alignment, a total of 18 differential amino acids were found, such as 19E>19K, 166D>116E, 167G>167W and so on. Among these differential amino acids, 16 registry Eplets were retrieved such as 19E>19K, 95I>95L, 113YD>113HD and so on.The amino acid substitution of 166DG>166EW, 70Q>70H, 67S>67Y, 94I>94T, 82LR>82RG, and 211G>211A may form new Eplets that have not been registered.The antigens of A11, A24, B15, B27 and B38 can be further subdivided into HLA narrow specific antigens. 【Conclusion】 It was found that there were HLA allele-specific antibodies in patients with PTR, suggesting that high-resolution typing of HLA-A, B should be carried out for these patients and platelet donors in HLA compatible transfusion of PTR.
