Effects of human albumin products on human stem cell culture
10.13303/j.cjbt.issn.1004-549x.2021.12.029
- VernacularTitle:人血白蛋白产品对人干细胞培养的影响
- Author:
Lu CHENG
1
;
Jun XU
1
Author Information
1. Shanghai RAAS Blood Products Co. Ltd., Shanghai 201401, China
- Publication Type:Journal Article
- Keywords:
plasma-derived human albumin;
recombinant human albumin;
human stem cells;
post-translational modification
- From:
Chinese Journal of Blood Transfusion
2021;34(12):1385-1387
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To compare the effects of human albumin products from different domestic manufacturers on human stem cell culture. 【Methods】 Human CD34+ cells were cultured by supplementing human albumin from different manufacturers to serum-free medium, and the expansion ratios of cells within 15 days were counted. Post translational modifications of human albumin products were studied by LC-MS. 【Results】 Supplementing plasma-derived human albumin(pd-alb) to serum-free medium, the expansion ratios of cells could reach up to 20.6±5.7, while the recombinant human albumin(rhAlb) resulted in no significant expansion within 15 days. LC-MS showed significant differences in post-translational modifications from different human albumin products. 【Conclusion】 Different human albumin products showed significantly different effects in the expansion of stem cells due to different sources, processes and stabilizers. Pd-alb products were better for stem cell culture than rhAlb products. There are significant differences between pd-albs and rhAlbs in post-translational modification, but whether these differences are related to stem cell expansion remains to be studied.
