Serological characteristics and genotype analysis of D epitope blood group of RhD variant individuals in Guangzhou
10.13303/j.cjbt.issn.1004-549x.2021.12.004
- VernacularTitle:广州地区RhD变异型个体D抗原表位血型血清学特征及基因型分析
- Author:
Boquan HUANG
1
;
Shuangshuang JIA
2
;
Jizhi WEN
2
;
Guangping LUO
2
;
Yanli JI
2
;
Huayou ZHOU
3
Author Information
1. The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China; Institute of Clinical Blood Transfusion, Guangzhou Blood Center
2. Institute of Clinical Blood Transfusion, Guangzhou Blood Center
3. The First School of Clinical Medicine, Southern Medical University, Guangzhou 510515, China
- Publication Type:Journal Article
- Keywords:
Rh blood group;
RhD variants;
epitopes of RhD antigen;
serology;
molecular biology;
MLPA
- From:
Chinese Journal of Blood Transfusion
2021;34(12):1290-1295
- CountryChina
- Language:Chinese
-
Abstract:
【Objective】 To explore the characteristics of the D antigen epitope of individuals with RhD variants and the genetic molecular mechanism of gene mutations in Guangzhou. 【Methods】 A total of 59 samples of RhD variants were collected from blood donors and hospitals in Guangzhou from January to August 2019. Serological characteristics of D epitopes were further analyzed using two kinds of monoclonal anti-D reagents and D epitope detection kits, and RHCE phenotypic typing was performed. QuickGene DNA extraction kit was used to extract the genomic DNA of the samples, and PCR-RFLP method was used to analyze the RHD gene zygote type. The RHD gene sequence was detected by multiple ligation-dependent probe amplification(MLPA) genotyping, and the RHD exon(1~10) Sanger sequencing was performed on the samples still in doubt after the above detection. DNAStar/SeqMan analysis software was used for comprehensive analysis. 【Results】 In this group of individuals with RhD variants in Guangzhou, 27.12%(16/59) were detected from blood donors [accounting for 0.007%(16/232 793) of blood donors in Guangzhou during the same period], and difficult samples of patients sent by hospitals for determination accounted for 72.88%(43/59). RHD genotype detection: 40.68%(24/59) were RHD*weak partial 15, 25.42%(15/59) were RHD* DⅥ.3 and 33.90%(20/59) were rare RHD variants [76.92%(10/13) were RhD variants with 2 different alleles]. Serological D-screen revealed a relatively fixed pattern of RHD*DⅥ.3 in anti-D antibody(clone: P3*212 23B10), while the others was negative. The phenotypic distribution of RhD variant CE was Ccee 38.98%(23/59), ccEe 35.59%(21/59), CcEe 25.42%(15/59). 【Conclusion】 Weak partial D15 and DⅥ.3 were the most common RhD variants in Guangzhou Han population, and DⅥ can be preliminarily identified by serological methods such as D-Screen anti-D reagent, while the remaining RhD variants can only be identified by molecular biological methods, and >95% of the RhD variants were C+ or E+ phenotypes.
