1.Bioweapon and bioterroism fighting
Journal of Medical and Pharmaceutical Information 2002;(2):1-4
There have more than toxic microbial and viruses in the list of bioweapon for the bioterrorism and biowar. The fights for bioterrorism and biodisaster are activities of society and community. Individuals and community have responsibilities to protect, fight, and reduce the damage timely and effectively to bioterrorism. The professional facilities are responsible for investigating, isolating the germs, toxics and implementing the effective solutions for management of outcomes of bioweapon.
Virology
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war
2.Comparison on throat swabs and respiratory tract aspirates for the detection of respiratory viruses from patients with pneumonia.
Ai-hua LI ; Weng-feng SHI ; Tie-gang ZHANG ; Min LV ; Meng CHEN ; Fang HUANG ; Jiang WU
Chinese Journal of Epidemiology 2013;34(10):1047-1047
Bodily Secretions
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virology
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Humans
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Pharynx
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virology
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Pneumonia
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diagnosis
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virology
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Respiratory System
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virology
3.Establishment PCR-based protocol for the detection of human papilloma virus in vaginal swab specimens
Journal Ho Chi Minh Medical 2005;9(1):49-53
The authors reported a PCR-based protocol for the detection of Human papilloma virus in vaginal swab speci
Papilloma
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Papilloma/virology
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Diagnosis
4.Result of serological and virological surveillance of Dengue fever/Dengue haemorrhagic fever in the Northern Vietnam, 2003
Journal of Preventive Medicine 2004;14(2):16-20
In the year 2003, 525 specimens seem to be infected with Dengue fever/Dengue haemorrhagic fever in the Northern Vietnam were sent to the National Institute of Hygiene and Epidemiology, those specimens with IgM, IgG antibodies against Dengue virus was 85 cases (16.2%). In 2003, the number of Dengue fever/Dengue haemorrhagic fever was sporadic distributed in some provinces. Owing to active prevention of Dengue fever/Dengue haemorrhagic fever, so Dengue virus type 2 discovered early in Ha Tay, Thanh Hoa, Nghe An and Tay Nguyen provinces; Dengue virus type 1 in Ha Noi, Nghe An, Tay Nguyen provinces and Dengue virus type 4 in Ha Tinh, Thanh Hoa, and Tay Nguyen provinces
Dengue
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epidemiology
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Serology
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Virology
5.Subcellular localization of severe fever with thrombocytopenia syndrome virus in macrophages.
Ying HAN ; Yi-Xiang LI ; Cong JIN ; Chuan LI ; Jing QU ; Fu-Shun ZHANG ; Quan-Fu ZHANG ; Mi-Fang LIANG ; Pei-Hong QIU
Chinese Journal of Experimental and Clinical Virology 2013;27(3):161-163
OBJECTIVETo study the subcellular localization of severe fever with thrombocytopenia syndrome virus (SFTSV) in macrophages and understand the replication and assembly mechanism of SFTSV in host cells.
METHODSUsing two types of human macrophage cell lines THP-1 and U937, the study analyzed the intracellular colocalization of SFTSV with Golgi apparatus and endoplasmic reticulum by immunefluorescence staining and confocal microscopy.
RESULTSSFTSV infected macrophage cell lines THP-1 and U937. Immunofluorescence staining showed that the SFTSV nuclear protein colocalized with Golgi apparatus and closely surrounded by endoplasmic reticulum in the perinuclear region.
CONCLUSIONThe results suggested that Golgi complex and endoplasmic reticulum are probably the sites for formation and maturation of SFTSV viral particles.
Bunyaviridae ; isolation & purification ; Cell Line, Tumor ; Endoplasmic Reticulum ; virology ; Fever ; virology ; Golgi Apparatus ; virology ; Humans ; Macrophages ; virology ; Thrombocytopenia ; virology
6.Human papillomavirus types and their related diseases.
Acta Academiae Medicinae Sinicae 2007;29(5):678-684
With the development of biomolecular technology, more human papillomavirus (HPV) types have been found to be related with various diseases. High-risk HPV 16, 18, 31, 33, 45, 52, and 61 have shown causal relationship with cancers of the cervix, vulva, vagina, penis, anus, oral cavity, and oropharynx. Low-risk HPV 6 and 11 have shown causal relationship with genital warts. HPV may also cause cancer of the larynx. However, the relationships between HPV and esophagus and lung cancers are still controversial.
Humans
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Neoplasms
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virology
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Papillomaviridae
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physiology
7.Progress in infection pathway and intracellular trafficking of adenovirus.
Wenfeng ZHANG ; Hongwei SHAO ; Hua HEL ; Shulin HUANG
Chinese Journal of Biotechnology 2014;30(6):864-874
The research on intracellular trafficking of adenovirus has been described mainly through observations of subgroup C adenoviruses in transformed cell lines. The basic elements of the trafficking pathway include binding to receptors at the cell surface, internalization by endocytosis, lysis of the endosomal membrane, escape to the cytosol, intracellular trafficking along microtubules, nuclear pore docking, and viral genome translocation into the nucleus. More than 80% of the adenovirus genome is delivered to the nucleus in a highly efficient manner in approximately 1 h. However, exceptions to this trafficking pattern have been noted, including: variations based on target cell type, cell physiology, and adenovirus serotype. This review summarizes mechanism of adenovirus infection pathway and intracellular trafficking, providinging a foundation for the development of clinical adenoviral vector.
Adenoviridae
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physiology
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Cell Membrane
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virology
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Cell Nucleus
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virology
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Cytoplasm
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virology
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Endocytosis
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Endosomes
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virology
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Genetic Vectors
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Humans
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Microtubules
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Virus Internalization
8.Virological studies of hospitalized cases of poliomyelitis.
VENZON E ; CHAN VF ; DE LA CRUZ L ; ZAPANTA L ; ESPIRITU-CAMPOS L
Journal of the Philippine Medical Association 0000;():0-
One hundred nine cases of poliomyelitis admitted to the Polio Pavilion of the San Lazaro Hospital in 1961 were studied. At least paired serum samples and in some instances, triple serum samples were collected from them. Ninety-one had matching stool specimensVirus isolation was done on the 91 stool samples and complement fixation test on the 129 paired or triple serum samplesThe pertinent findings were as follows: (1) only half of the cases clinically recognized as poliomyelitis could be confirmed by either or both tests employed; (2) about one-third of the agents isolated from stools were non-polio; (3) the most prevalent type of poliovirus causing clinically apparent illness is type 1. The least common is type 2; (4) poliomyelitis is still an infantile type of disease in this country since the majority of the confirmed cases occurred in the age group 0-23 months; (5) maternal antibodies may cease to be protective even before an infant reaches 4 months of age; (6) during the period of study, cases of poliomyelitis were more or less evenly distributed throughout the year. (Summary)
Virology, Viruses, Infants, Newborn, Infant
9.Susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to infection with bat Japanese encephalitis virus isolates.
Shan LIU ; Qionghua ZHANG ; Junhua ZHOU ; Shouyi YU ; Xueli ZHENG ; Qing CHEN
Journal of Southern Medical University 2012;32(4):515-518
OBJECTIVETo evaluate the susceptibility of Aedes albopictus and Culex pipiens quinquefasciatus to oral infection with bat Japanese encephalitis virus isolates (GD1 and HN2 strains).
METHODSAedes albopictus and Culex pipiens quinquefasciatus were infected orally by GD1 and HN2 strains of bat Japanese encephalitis virus. TaqMan real-time PCR was used to detect the virus and monitor the changes in the viral loads in Aedes albopictus and Culex pipiens quinquefasciatus at a 2-day interval, starting from 4 days till 20 days after the infection.
RESULTSThe infected Aedes albopictus and Culex pipiens quinquefasciatus were found positive for the Japanese encephalitis virus from day 4 to day 20. Both Aedes albopictus and Culex pipiens quinquefasciatus were susceptible to infection by GD1 and HN2 strains, but the latter showed a greater susceptibility. The HN2 strain virus appeared to have a greater virulence than the GD1 strain.
CONCLUSIONAedes albopictus and Culex pipiens quinquefasciatus can carry GD1 and HN2 strains of bat Japanese encephalitis virus isolates.
Aedes ; virology ; Animals ; Chiroptera ; virology ; Culex ; virology ; Disease Susceptibility ; Encephalitis Virus, Japanese ; isolation & purification