1.The Bacteriological Survey of the Main Streams in Kyong-Ju City: on Samonellae.
Korean Journal of Preventive Medicine 1989;22(2):268-275
The studies on the standard plate count, most probable number(MPN) and isolation of the Enterobacteriaceae, especially Salmonella species from stream waters of Kyong-Ju city were carried out from June to July, 1988. The relationship between water temperature, weather and standard plate count were observed. The locations where all of the MPN was higher than 1,600 were Mun-chon bridge, Wol-song bridge, Go-hun bridge and the area adjoining the Hyong-san river. The higher the water temperature, the more the viable counts without rain. Bacteriological survey of the Salmonella species was performed with the total 80 specimens isolated from 16 areas which are the main streams in Kyong-Ju city. The author identified 5 strains(2.36%) of Salmonella typhi, 5 strains(2.3%) of Salmonella paratyphi, 2 strains(1.42%) of Salmonella paratyphi B, 63 strains(29.72%) of other Salmonella groups by biochemical tests and serotyping tests with slide agglutination.
Agglutination
;
Enterobacteriaceae
;
Gyeongsangbuk-do*
;
Rain
;
Rivers*
;
Salmonella
;
Salmonella paratyphi A
;
Salmonella paratyphi B
;
Salmonella typhi
;
Serotyping
;
Water
;
Weather
2.Enteric fever: an appraisal of clinical laboratory and therapeutic aspects.
Acta Medica Philippina 0000;():0-
Ninety-six episodes of enteric fever due to Salmonella paratyphi A and Salmonella typhi were studied. Eighty-two per cent of cases were due to S. paratyphi A, 14 to S. typhi and 4 were mixed infections. There was no observer in-vitro resistance to chloramphenicol and co-trimoxazole among the isolates. Clinical jaundice was observed in 19 per cent of cases and correlated with the frequent occurrence of abnormal elevation of serum bilirubin, alkaline phosphatase, and SGOT/SGPT. Blood cultures were most helpful in establishing the diagnosis while the Widals test was of limited usefulness and showed a low sensitivity as diagnostic tool. Defervescence was noted on 4.4 days with chloramphenicol, 4.4 days with co-trimoxazole, and 6.2 days with thiamphenicol. There were three treatment failures with chloramphenicol, one with co-trimoxazole, and two with thiamphenicol. There was no relapse observed with co-trimoxazole therapy compared to 4 relapses with chloramphenicol and 1 on thiamphenicol and another on ampicillin therapy. Complications were encountered in 23 patients including gastrointestinal hemorrhage in 19 cases, (4 of whom required transfusion), intestinal perforation, toxemia, and acute renal failure occurring in one patient each. Hemolytic crisis in one patient and agranulocytosis in another, presumed to be side effects of therapy, were observed. Four patients died; two because of massive gastrointestinal hemorrhage, one due to progressive toxemia and another due to acute renal failure. (Summary)
Salmonella Paratyphi A, Salmonella Typhi, Chloramphenicol, Thiamphenicol
3.Literature Review and Clinical Presentation of Cervical Spondylitis Due to Salmonella Enteritidis in Immunocompetent.
Zhi Yun FENG ; Fang GUO ; Zhong CHEN
Asian Spine Journal 2014;8(2):206-210
A 48-year-old woman was presented to our clinic with some fever and neck pains for about one month. Based on the symptoms and results of image, an empirical diagnosis of tuberculous cervical spondylitis was made. The pain was not significantly decreased after anti-tuberculosis therapy. And, 3 weeks later, she was re-admitted to our hospital for the unbearable pain. An exploration of the C4/5 by the anterior medial approach was recommended to evaluate the germ and debridement. Bacteriological tests showed that the pathogen was Salmonella Enteritidis. The pain was relieved significantly after operation and sensitive antibiotic treatments. Infections with Salmonella Typhi or Salmonella Paratyphi have been well-documented, while there are few reports of cervical spondylitis caused by Salmonella Enteritidis. We reported a case of a healthy woman with whom pyogenic cervical spondylitis of Salmonella Enteritidis was corroborated and treated and reviewed according to previous reports about spondylitis caused by Salmonella Enteritidis in the literature.
Cervical Vertebrae
;
Debridement
;
Diagnosis
;
Female
;
Fever
;
Humans
;
Middle Aged
;
Neck Pain
;
Salmonella enteritidis*
;
Salmonella paratyphi A
;
Salmonella typhi
;
Salmonella*
;
Spondylitis*
4.Efficacy and clinical trials of Salenvac-T, bivalent killed vaccine containing Salmonella Enteritidis and Salmonella Typhimurium.
Youngjae CHO ; Zheng Wu KANG ; Kyung Soo KANG ; Seunghwan JEONG ; Hee Jun YOON ; Seungwon SUH ; Tae Wook HAHN
Korean Journal of Veterinary Research 2013;53(1):43-48
Commercial bivalent killed Salmonella vaccine Salenvac-T has been used in several countries in order to prevent salmonellosis with Salmonella enterica serovars Enteritidis (SE) and Typhimurium (ST) in poultry. However, this vaccine has not been used in poultry farms in South Korea. In this study, we evaluated the efficacy of Salenvac-T vaccine to protect against the challenge of virulent SE and ST, and the effect of the vaccine on egg production and mortality in layer hens. The colonization of liver, spleen and cecum with challenged SE and ST was reduced in vaccinated chickens compared with that of unvaccinated control group. The twice vaccination with Salenvac-T induced elevated antibody responses against both SE and ST detected by enzyme-linked immunosorbent assay (ELISA). The higher average hen-day production was observed in the vaccinated layer hens than in the unvaccinated layer hens without significance. The average mortality was lower in the vaccinated layer hens during the experiment period. The antibody responses to both SE and ST were persistently detected in the vaccinated layers. In summary, vaccination with Salenvac-T reduces colonization of internal organs and induces good antibody responses, thereby results in higher performance and lower egg contamination with SE and ST in layer hens.
Antibody Formation
;
Cecum
;
Chickens
;
Colon
;
Enzyme-Linked Immunosorbent Assay
;
Liver
;
Ovum
;
Poultry
;
Republic of Korea
;
Salmonella
;
Salmonella enterica
;
Salmonella enteritidis
;
Salmonella Infections
;
Salmonella typhimurium
;
Spleen
;
Vaccination
6.Multidrug-resistant Salmonella typhimurium and Salmonella enteritidis Identified by Multiplex PCR in Korea.
Soo Jin YANG ; Kyoung Yoon PARK ; Keun Seok SEO ; Thomas E BESSER ; Yoon Ho KOOK ; Han Sang YOO ; Yong Ho PARK
Journal of the Korean Society for Microbiology 2000;35(5):366-366
No Abstract Available.
Korea*
;
Multiplex Polymerase Chain Reaction*
;
Salmonella enteritidis*
;
Salmonella typhimurium*
;
Salmonella*
7.Computed tomography features in enteric fever.
Tiffany HENNEDIGE ; Doris S BINDL ; Ambika BHASIN ; Sudhakar K VENKATESH
Annals of the Academy of Medicine, Singapore 2012;41(7):281-286
<b>INTRODUCTIONb>Enteric fever is a common infection in endemic areas; however, there are few reports describing the computed tomography (CT) manifestations of enteric fever. We aim to describe and illustrate CT findings in enteric fever in this study.
<b>MATERIALS AND METHODSb>A retrospective search of medical records in our institute for patients with positive blood cultures for Salmonella typhi, and Salmonella paratyphi organisms yielded 39 cases. Among these patients, 12 had undergone a CT study of the abdomen. The CT images, laboratory and clinical findings of these 12 patients were reviewed.
<b>RESULTSb>The most common clinical presentation was fever (100%). Typical features of gastroenteritis were present in only over half of the patients. Liver function tests were abnormal in all patients. The most common abdominal manifestations on CT were the presence of mesenteric lymphadenopathy and splenomegaly (75%). Other features were circumferential small bowel wall thickening (58.3%) and free intraperitoneal fluid (50%). Three patients were found to have complications; one with bleeding from terminal ileal ulceration, another with an ileal perforation and the third with renal abscess formation.
<b>CONCLUSIONb>CT is useful in evaluating enteric fever in patients with severe forms of presentation, a longer clinical course or less specific symptoms. Although the imaging features overlap with other abdominal infections, when combined with clinical features, travel history to endemic areas and presence of transaminitis, the diagnosis of enteric fever should be considered. CT in particular, is useful for the detection of complications such as perforation, bleeding and abscess formation.
Abscess ; diagnostic imaging ; Adolescent ; Adult ; Aged ; Ascitic Fluid ; diagnostic imaging ; Child ; Child, Preschool ; Female ; Humans ; Intestine, Small ; diagnostic imaging ; Lymphatic Diseases ; diagnostic imaging ; Male ; Middle Aged ; Paratyphoid Fever ; diagnostic imaging ; Radiography, Abdominal ; Retrospective Studies ; Salmonella paratyphi A ; Salmonella paratyphi B ; Salmonella typhi ; Splenomegaly ; diagnostic imaging ; Tomography, X-Ray Computed ; Typhoid Fever ; diagnostic imaging
8.Protection Against Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Enteritidis Infection in Layer Chickens Conferred by a Live Attenuated Salmonella Typhimurium Strain.
Immune Network 2015;15(1):27-36
In the present study, we investigated the protection conferred by a live attenuated Salmonella enterica serovar Typhimurium (ST) strain against Salmonella Typhimurium, Salmonella Gallinarum (SG), and Salmonella Enteritidis (SE) infection in layer chickens. Birds were orally primed with the attenuated ST strain at 7 days of age and then boosted at 4 weeks post prime immunization (PPI). Sequential monitoring of plasma IgG and mucosal secretory IgA (sIgA) levels revealed that inoculation with ST induced a significant antibody response to antigens against ST, SE, and SG. Moreover, significant lymphoproliferative responses to the 3 Salmonella serovars were observed in the immunized group. We also investigated protection against virulent ST, SE, and SG strain challenge. Upon virulent SG challenge, the immunized group showed significantly reduced mortality compared to the non-immunized group. The reduced persistence of the virulent ST and SE challenge strains in the liver, spleen, and cecal tissues of the immunized group suggests that immunization with the attenuated ST strain may not only protect against ST infection but can also confer cross protection against SE and SG infection.
Antibody Formation
;
Birds
;
Chickens*
;
Cross Protection
;
Immunization
;
Immunoglobulin A, Secretory
;
Immunoglobulin G
;
Liver
;
Mortality
;
Plasma
;
Salmonella enterica
;
Salmonella enteritidis*
;
Salmonella typhimurium*
;
Salmonella*
;
Spleen
9.Is the Widal Test Still Useful?.
Sun E KIM ; Think You KIM ; Ile Kyu PARK ; Jung Oak KANG ; Tae YEAL
Korean Journal of Clinical Pathology 1999;19(2):215-221
BACKGROUND: Widal test has been one of the most important diagnostic tests for typhoid and is still widely used. Widal test has been useful diagnostic tool for typhoid in endemic areas, while it has been largely abandoned in developed countries. Since 1990, occurrance of typhoid has been markedly decreased in Korea, we studied diagnostic usefulness and criteria of Widal test from 1990 to 1997. METHODS: Using rapid slide titration method (Stained Salmonella suspensions, Murex Biotech Ltd., Dartford, England), the Widal test was done in 116 nontyphoid salmonellosis patients, 75 patients with proven typhoid fever, and 173 cases of clinically suspected typhoid fever patients. Stastical analysis was done with discriminant analysis in culture proven salmonellosis. RESULTS: Fifty-four culture proven cases of Salmonella paratyphi (S. paratyphi) A and S. paratyphi B showed no significant cutoff value in O antibodies. Salmonella typhi (S. typhi) O titer at the 1:160 and above showed lower sensitivity (37.3% vs. 69.3%) and specificity (91.4% vs. 93.1%) compared to S. typhi H titer at the 1:320 and above in diagnosis of culture proven cases of typhoid. We applied D (0.01xH titer+0.001xO titer-1.635) score which result from discriminant analysis. Positive D score (> or =0.21) showed sensitivity of 72% and specificity of 92.2% in culture proven cases of typhoid. In clinically suspected patients, positive D score showed 39.3% of sensitivity. CONCLUSIONS: We concluded that the Widal test for O antibodies of S. paratyphi A and S. paratyphi B is not useful for diagnosis of paratyphoid fever. In the present study S. typhi H antibodies are more diagnostic than S. typhi O antibodies. We appled D score and positive D score showed increased sensitivity of Widal test than application of O antibody titer.
Antibodies
;
Developed Countries
;
Diagnosis
;
Diagnostic Tests, Routine
;
Humans
;
Korea
;
Paratyphoid Fever
;
Salmonella
;
Salmonella Infections
;
Salmonella paratyphi A
;
Salmonella typhi
;
Sensitivity and Specificity
;
Suspensions
;
Typhoid Fever
10.Investigation of antigen related to the in vitro invasiveness of salmonella typhimurium through the Madin-Darby canine kidney(MDCK) epithelial cell monolayer.
Chung Gyu PARK ; Eung Soo HWANG ; Ju Young SEOH ; Yoon Hoh KOOK ; Chang Yong CHA
Journal of the Korean Society for Microbiology 1991;26(6):553-562
No abstract available.
Epithelial Cells*
;
Salmonella typhimurium*
;
Salmonella*