1.Osteocalcin Response to Calcium Restricted Diet for the Selective Therapy of Hypercalciuria.
Young Tae MOON ; Seung Hwan YOON
Korean Journal of Urology 2000;41(4):516-520
No abstract available.
Calcium*
;
Diet*
;
Hypercalciuria*
;
Osteocalcin*
2.The effect of 1,25(OH)D3 deficiency in the secondary dentin formation and mineralization and caries of the mice.
Hong LIU ; Liu-cai YANG ; Deng-shun MIAO
West China Journal of Stomatology 2010;28(6):599-606
OBJECTIVETo determine the role of 1,25(OH)2D3 on the secondary dentin formation and mineralization of the mice.
METHODSThe differences of the mandible mineralization between the wild-type and 1-alpha-hydroxylase gene knockout mice at 6 weeks old were assessed by hematoxylin-eosin (HE) staining, immunohistochemistry, alkaline phosphatase (ALP) histochemistry staining.
RESULTSThe ratio of caries were increased significantly, while the secondary dentin was reduced significantly, the deposition of type I collagen and osteocalcin on the secondary dentin of occlusion surface was decreased significantly, but the deposition of the Biglycan on the dentin was increased significantly, the active of ALP on the odontoblasts were reduced significantly in 1-alpha-hydroxylase gene knockout mice compared to that in the wild-type littermates.
CONCLUSION1,25(OH)2D3 deficiency lead to a defect in the secondary dentin formation and mineralization and caries of the mice.
Animals ; Dentin ; Dentin, Secondary ; Mandible ; Mice ; Osteocalcin
3.Effect of nano-granule titanium films on synthesis of osteoblasts.
Kai BA ; Jing ZHANG ; Hu WANG ; Yuan-yuan LIU ; Zhen-yu YANG ; Ming-xia LI ; Wei LI ; Jing-wei MIAO
West China Journal of Stomatology 2009;27(6):592-594
OBJECTIVETo compare the synthetic ability of osteoblasts on the surface of different nano-granule titanium films and investigate the correlation between nanophase titanium films and cellular biocompatibility.
METHODSFour different nano-granule titanium films were produced by direct current magnetron sputtering, at ambient, 100 degrees C, 250 degrees C, 380 degrees C substrate temperature, respectively. Rat osteoblasts were seeded on the surface of four treated groups of titanium film samples and non-treated Ti sample(control group). The production of osteocalcin (OC) in all five groups were detected by using double antibody sandwich enzyme-linked immunosorbent assay.
RESULTSThe production of OC increased gradually from day 7 to day 14 in all groups. In the control group, it showed significant differences with other five groups on day 7. On day 14, the production of OC in 100 degrees C group was the highest, and it showed significant differences with 380 degrees C, control group and blank group. In 250 degrees C group, the production of OC also showed significant differences with 380 degrees C, control group and blank group (P < 0.05).
CONCLUSIONTitanium with nano-modified surface had good biocompatibility and different nano-granule titanium films could affect the synthesis of osteoblasts.
Animals ; Osteoblasts ; Osteocalcin ; Rats ; Surface Properties ; Titanium
4.The Effects of Hormon Replacement Therapy on Serum Osteocalcin, Serum Calcium, Serum Alkaline Phosphatase, and Urine Calcium of Postmenopausal Women.
Cheon June LEE ; Heung Yeol KIM ; Un Dong PARK
Korean Journal of Obstetrics and Gynecology 1997;40(12):2733-2740
No abstract available.
Alkaline Phosphatase*
;
Calcium*
;
Female
;
Humans
;
Osteocalcin*
5.Bone functions as a novel endocrine organ in energy metabolism.
Xiang CHEN ; Hao-ming TIAN ; Fu-xing PEI ; Xi-jie YU
Chinese Medical Journal 2012;125(22):4117-4121
6.Effects of salvianolic acid B on osteogenic differentiation of human periodontal ligament cells.
West China Journal of Stomatology 2016;34(5):468-473
OBJECTIVEThis study investigated the effects of salvianolic acid B (Sal B), a major bioactive component of the Chinese medicine salvia miltiorrhiza, on osteogenic differentiation of human periodontal ligament cells (hPDLCs).
METHODSThird passage PDLCs were used in this experiment. Methyl thiazolyl tetrazolium (MTT) method was employed to observe the effects of different Sal B concentrations on proliferation activity of hPDLCs. Alkaline phosphatase (ALP) activity and mineralization capability were measured, and mRNA expression of osteocalcin (OCN) was detected to investigate the effects of Sal B on osteogenesis of hPDLCs.
RESULTSSal B did not influence the viability of hPDLCs. The ALP activity and OCN mRNA expression levels of hPDLCs were both significantly improved (P<0.05) under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) compared with those in OIM group. Moreover, the number of mineralized nodules formed by hPDLCs were considerably higher under treatment with different Sal B concentrations (0.5, 1, and 5 μmol·L⁻¹) than that in the OIM group.
CONCLUSIONSAppropriate Sal B concentration can improve the osteogenic differentiation of hPDLCs.
Benzofurans ; Cell Differentiation ; Cells, Cultured ; Humans ; Osteocalcin ; Osteogenesis ; Periodontal Ligament
7.Beta-catenin, Frizzled-2, BMP-4, Osteopontin, Osteocalcin, and Osteonectin Expression in Calcifying Basal Cell Carcinoma.
Mi Ok DO ; So Young KIM ; You Won CHOI ; Ki Bum MYUNG ; Hae Young CHOI
Korean Journal of Dermatology 2007;45(7):666-672
BACKGROUND: Although basal cell carcinoma (BCC) is the most common human skin tumor, with calcification reportedly taking place in about 20% of all BCC cases, the pathogenesis of calcification in BCC has not yet been studied. OBJECTIVE: The purpose of this study was to evaluate factors related to the pathogenesis of calcification in BCC. METHODS: We performed immunohistochemical staining for beta-catenin, frizzled-2, BMP-4, osteopontin, osteocalcin, and osteonectin using frozen skin tissue from 15 cases of BCC with calcification and 11 cases of BCC without calcification. RESULTS: The expression of beta-catenin showed positive in 14 of the 15 cases in BCC with calcification, but negative in all 11 cases of BCC without calcification. The expression of frizzled-2 was observed in 14 of the 15 cases in BCC with calcification, and in 10 of the 11 cases in BCC without calcification. The difference did not reach statistical significance (p=0.236). The expression of BMP-4 was observed in all 26 samples of BCC, but the intensity of expression did not reach statistical significance between the two groups (p=0.293). Furthermore, osteopontin and osteocalcin showed no statistical significance between two the groups (p=0.567, p=0.401). The expression of osteonectin was observed in all of the BCC cases, and was stronger in BCC with calcification than in BCC without calcification (p=0.042). CONCLUSION: We suggest that the calcification in BCC might be related to the increase of beta-catenin expression and that osteonectin might also influence the process of calcification in BCC.
beta Catenin*
;
Carcinoma, Basal Cell*
;
Humans
;
Osteocalcin*
;
Osteonectin*
;
Osteopontin*
;
Skin
8.Effects Of Chitosan On Human Osteoblasts.
Ki Hong KIM ; Young Ju PARK ; Jun Woo PARK ; Yong Chan LEE ; Byoung Ouck CHO ; Byoung Keun AHN
Journal of the Korean Association of Oral and Maxillofacial Surgeons 2002;28(4):280-285
As the result of the study concerning "bone inducibility of chitosan", 1. "BMP-2"was observed mainly through the test when the "osteoblast"is exposed to the "chitosan". The expression of BMP-2 was 542.63 times compared to control after 2 hours exposure and it was maintained 16.60 times till 24 hours. 2. The expression of BMP-4 was decreased compared to control during exposure. 3. The expression of BMP-7 revealed two peaks during exposure. 4. The expression of osteocalcin was increased in early phase, and then decreased. Although it is not clear whether the "chitosan"is clinically effective material as a "bone induction material", we could say that it has a function for bone induction. Further detailed study will be required.
Bone Morphogenetic Protein 7
;
Chitosan*
;
Humans*
;
Osteoblasts*
;
Osteocalcin
9.Adenovirus-Mediated Toxic Gene Therapy Using Cytosine Deaminase and Osteocalcin Promoter for the Treatment of Prostate Cancer.
Hong Seok PARK ; Jae Hyun BAE ; Du Geon MOON ; Hyun Yee CHO ; Chinghai KAO ; Thomas A GARDNER ; Jun CHEON
Korean Journal of Urology 2000;41(12):1437-1444
No abstract available.
Cytosine Deaminase*
;
Cytosine*
;
Genetic Therapy*
;
Osteocalcin*
;
Prostate*
;
Prostatic Neoplasms*
10.Effects of Weight-Bearing Exercise on Bone Metabolism in College Women.
Ju Sung KIM ; Myung Hee KIM ; Jae Shin SHIN
Journal of Korean Academy of Nursing 2004;34(5):760-770
PURPOSE: This study was done to identify the effects of weight-bearing exercise(WBE) on bone metabolism. METHOD: WBE was performed for 12 weeks by healthy college women. Bone-related parameters were measured four times during this period by evaluating the immunoradiometric assay and enzyme immunoassay. Bone mineral densities(BMDs) were measured by dual energy x-ray absorptiometry before and after the WBE program. Data was analyzed using t-test, paired t-test, x2-test, and repeated measures ANOVA. RESULT: Osteocalcin, a bone formation marker, increased more in the experimental group than in the control group based on the interaction between time and group(F=3.29 p=.024). Little difference between the two groups was found for the other parameters: urinary deoxypyridinoline, insulin-like growth factorI, parathormone, serum calcium, and serum phosphorus without showing any time interaction between the groups. The femoral trochanter BMD rose in the experimental group while that of the control group fell, showing a significant difference for BMD(t=3.06 p=.005). However, there was no significant difference between the two groups for changes in BMD of the forearm, lumbar spine, femoral neck, and femoral ward's triangle. CONCLUSION: These findings supported the WBE is beneficial for increasing bone formation in college women and long-term application is needed to substantiate the effects of WBE as a intervention in promotion of bone-health.
Absorptiometry, Photon
;
Adult
;
*Bone Density
;
*Exercise
;
Female
;
Humans
;
Osteocalcin/blood