3.Two Cases of Epithelioid Sarcoma with Immunohistochemical Study.
Duk Kyu CHUN ; Kwang Hyun CHO ; Seong Hoe PARK
Annals of Dermatology 1992;4(1):26-31
Two typical cases of epithelioid sarcoma were examined by immunohistochemical stain using antibodies to epithelial membrane antigen, carcinoembryonic antigen, vimentin and cytokeratin. Both cases showed positive reactivity for the four kinds of antibodies. These results point to the fact that epithelioid sarcoma simultaneously expresses epithelial markers and characteristic mesenchymal phenotypes. Epithelioid sarcoma appears to be a tumor derived from a multipotential mesenchymal cell with multidirectional differentiation.
Antibodies
;
Carcinoembryonic Antigen
;
Keratins
;
Mucin-1
;
Phenotype
;
Sarcoma*
;
Vimentin
4.Microcystic Meningioma - Unusual Variant of Meningiomas.
Jun Hyung CHO ; Kook Hee YANG ; Ho Yeal ZHANG ; Jeong Hae KIE
Journal of Korean Neurosurgical Society 2003;34(4):382-385
Microcystic meningioma is a rare variant of meningiomas. This unusual variant was originally described by Masson, who labeled it "humid". The computed tomographic scan or magnetic resonance images of these tumors resemble those of a glial or metastatic tumor with cystic or necrotic changes. There is no definitive method for differentiating cystic meningiomas from these more common tumors. But immunohistochemically, they share a similar pattern of positive staining for epithelial membrane antigen and vimentin with other meningiomas. Our case was a 34-year-old woman with a tumor mass on the right frontal area. She was admitted to hospital because of generalized tonic seizure. Grossly all of the tumor could be removed, and histopathologically this tumor was revealed to be a microcystic meningioma.
Adult
;
Female
;
Humans
;
Meningioma*
;
Mucin-1
;
Seizures
;
Vimentin
5.Immunohistochemical Study on Acral Lentiginous Melanoma.
You Chan KIM ; Min Geol LEE ; Sang Ho CHO ; Sung Whan CHOE ; Hyang Joon PARK ; Yong Woo CINN
Korean Journal of Dermatology 2002;40(6):620-625
BACKGROUND: Although clinicopathologic characteristics of acral lentiginous melanoma (ALM) is well established, immunohistochemical study on ALM has rarely been reported. OBJECTIVE: Our purpose is to evaluate the usefulness of several immune markers in the diagnosis of ALM. METHODS: An immunohistochemical study was performed on paraffin sections of 20 ALMs using S-100 protein, HMB-45, vimentin, epithelial membrane antigen (EMA), and CAM 5.2. RESULTS: 1. Nineteen (95%) and 16 (80%) out of 20 ALM showed reactivity with S-100 protein and HMB-45, respectively. 2. Melanin bleaching was useful for diagnosing heavily pigmented ALM using both S-100 protein and HMB-45. 3. The immunoreactivity of S-100 protein and HMB-45 did not correlate with tumor thickness or level of invasion of ALM. The intensity of HMB-45 correlated well with the melanin content. 4. One and 2 out of 20 cases stained focally with EMA and CAM5.2 respectively, but these cases stained also with HMB-45 and/or S-100 protein. CONCLUSION: S-100 protein and HMB-45 were relatively sensitive markers for diagnosing ALM. Despite the occasional positivity for the epithelial markers in ALM, all epithelial marker-positive cases stained also with HMB-45 and/or S-100 protein. Therefore, S-100 protein and HMB-45 are very useful markers for diagnosing ALM.
Biomarkers
;
Diagnosis
;
Melanins
;
Melanoma*
;
Mucin-1
;
Paraffin
;
S100 Proteins
;
Vimentin
6.Expression of MUC1 and MUC4 and Its Prognostic Significance in Non-Small Cell Lung Carcinoma.
Ji Min JEON ; Hye Won LEE ; Ji Young PARK ; Hye Ra JUNG ; Ilseon HWANG ; Sun Young KWON ; Mi Sun CHOE ; Yu Na KANG ; Sang Pyo KIM ; Sang Sook LEE ; Won Il CHOI ; Kun Young KWON
Korean Journal of Pathology 2010;44(4):397-403
BACKGROUND: Mucin (MUC)1 and MUC4 (MUC1, 4) are high molecular weight glycoproteins expressed in normal and malignant epithelial cells, and these expressions are related to the prognosis of some carcinomas. In non-small cell lung carcinoma (NSCLC), the relationship between MUC1, 4 expressions and their prognostic significance is not well known. We evaluated these relationships in a series of NSCLC: 1) between MUC1, 4 expression levels and histologic subtypes, and 2) between high expression of MUC1, 4 and their prognostic significance. METHODS: We performed immunohistochemical staining for MUC1, 4 in paraffin-embedded tissues from 165 NSCLC cases arranged in a tissue microarray. RESULTS: We found a significant correlation between MUC1, 4 expressions and NSCLC histologic subtypes (p < 0.05). High MUC1 expression was characteristic of adenocarcinoma. Low MUC1, 4 expressions were characteristic of squamous cell carcinoma. In adenocarcinoma, we found significant association between diffuse MUC1 expression and short patient survival (p = 0.005). In squamous cell carcinoma, diffuse MUC4 expression showed long patient survival trend (p = 0.128). CONCLUSIONS: MUC1, 4 expression levels were significantly correlated with NSCLC histologic subtypes. Diffuse MUC1 expression was significantly associated with shortened survival in NSCLC patients, especially in adenocarcinoma.
Adenocarcinoma
;
Carcinoma, Squamous Cell
;
Epithelial Cells
;
Glycoproteins
;
Humans
;
Lung
;
Molecular Weight
;
Mucin-1
;
Mucin-4
;
Mucins
;
Prognosis
7.A Case of Palisaded Encapsulated Neuroma of the Lower Lip.
Young Hun CHO ; Ju Hee LEE ; Dong Sik BANG ; You Chan KIM
Korean Journal of Dermatology 2002;40(12):1552-1556
Palisaded encapsulated neuroma(PEN) is a rare intraneural neuroma. It usually occurs as a solitary, asymptomatic, skin-colored papule, and commonly affects the butterfly area of the face of middle-aged adults. However, it rarely involves oral mucosa including lip and should be differentiated from mucosal neuromas which generally occur as multiple small nodules of the lips and the anterior part of the tongue. We report a case of PEN occurring on the lower lip in a 33-year-old male. Histopathologically, it appeared as a well-circumscribed, encapsulated round nodule in the dermis. The nodule was composed of well-developed fascicles of wavy spindle cells separated by a loose matrix. Nuclear palisades were ill defined. On immunohistochemical staining, most tumor cells of the nodule were positive for S-100 protein and about 30% of tumor cells were positive for neural filament, but the capsule was negative for them. Epithelial membrane antigen was focally and discontinuously reactive on the capsule of the nodule.
Adult
;
Butterflies
;
Dermis
;
Humans
;
Lip*
;
Male
;
Mouth Mucosa
;
Mucin-1
;
Neuroma*
;
S100 Proteins
;
Tongue
8.A Case of Malignant Eccrine Poronta.
Joung Soo KIM ; Young Suck RO ; Chan Kum PARK
Korean Journal of Dermatology 1998;36(4):717-721
Malignant eccrine poroma is a rare malignant tumor arising from the intraepidermal ductal portion of the eccrine sweat gland, which develops either spontaneously or from a long-standing benign eccrine poroma. It usually affects older people and is located most commonly on the lower extremities. We describe a typical case of malignant eccrine poroma developing from benign eccrine poroma, which had been present for 10 years or more, with subsequent regional lymph node metastasis. A biopsy specimen revealed many well-defined intraepidermal nests of poroma cells with dermal invasion and lymphatic involvement of tumor cells. Immunohistochemical studies showed staining with cocktail cytokeratin, carcino-embryonic antigen, epithelial membrane antigen in intracellular vacuoles of tumor cells and early embryonic stages of eccrine duct formation. Local excision with postoperative adjuvant radiation therapy showed only a temporary response.
Biopsy
;
Eccrine Porocarcinoma
;
Keratins
;
Lower Extremity
;
Lymph Nodes
;
Mucin-1
;
Neoplasm Metastasis
;
Poroma
;
Sweat Glands
;
Vacuoles
9.Histologic Variations and Immunohistochemical Features of Metastatic Clear Cell Renal Cell Carcinoma.
Cheol LEE ; Jeong Whan PARK ; Ja Hee SUH ; Kyung Han NAM ; Kyung Chul MOON
Korean Journal of Pathology 2013;47(5):426-432
BACKGROUND: Due to advancements in treatment of metastatic and advanced renal cell carcinoma (RCC), it has become increasingly important to diagnose metastatic RCC and the specific subtype. In this study, we investigated the diverse histologic features of metastatic clear cell renal cell carcinoma (CCRCC) cases in comparison with corresponding primary lesions. METHODS: We identified 119 metastatic CCRCC cases from 81 corresponding primary lesions diagnosed between 1995 and 2010 and evaluated the diverse histologic and immunohistochemical features of these lesions. RESULTS: A total of 44 primary lesions (54.3%) had a non-clear cell component in addition to a typical clear cell component. Of the 119 metastatic lesions, 63 lesions (52.9%) contained a non-clear cell component, and 29 metastatic lesions were composed of a non-clear cell component only. Rhabdoid features were the most frequent non-clear cell histology among the metastatic lesions. Metastatic CCRCCs mainly showed positive CD10 and epithelial membrane antigen staining and negative cytokeratin 7 staining. CONCLUSIONS: Metastatic CCRCC commonly showed a variety of histologic features. If there is a difficulty to diagnose metastatic CCRCC due to a variety of histologic features or small biopsy specimen, histologic review of the primary lesion and immunohistochemical analysis can help determine the correct diagnosis.
Biopsy
;
Carcinoma, Renal Cell*
;
Cellular Structures
;
Immunohistochemistry
;
Keratin-7
;
Mucin-1
;
Neoplasm Metastasis
10.A Case of palisaded, Encapsulated Neuroma.
Seok Jin HONG ; Sam Hyeong KIM ; Hoon KANG ; Sook Ja SON ; Hee Jin JANG
Korean Journal of Dermatology 1998;36(1):173-176
Palisaded, encapsulated neuroma is characterized clinically by a solitary, slowly-growing and dome-shaped papule or nodule, usually accompanied by telangiectasia on the surface, and histopathologically by an encapsulated nodule in the dermis and the palisading arrangement of nuclei. A 57-year-old female patient presented with a 1cm-sized, solitary nodule on the right ala nasi which had been present for about 5 years. The nodule had a tendency to slow growth and it became a polypoid nodule. Telangiectasia was shown on the surface of the lesion. Histopathlogical findings showed a well-defined and encapsulated nodule in the dermis, composed of spindle cells with basophilic and plump nuclei in a palisading fashion. On immunohistochemical staining, the tumor cells of the nodule were positive for S-100 protein, while the capsule of the nodule was negative for S-100 protein. Epithelial membrane antigens were focally positive only on the capsule of the nodule. We report herein a case of palisaded, encapsulated neuroma, one case of which has been reported in Korea.
Basophils
;
Dermis
;
Female
;
Humans
;
Korea
;
Middle Aged
;
Mucin-1
;
Neuroma*
;
S100 Proteins
;
Telangiectasis