1.A Study on the Methylation of p 16 Gene Promotor in the Uterine Cervical Neoplasia.
Dong Han BAE ; Min Kwan KIM ; Chung Il LEE ; Chang Jin KIM
Korean Journal of Obstetrics and Gynecology 2000;43(9):1525-1532
No abstract available.
Methylation*
2.Advance of study on demethylation in leukemia - review.
Bei-Ming SHOU ; Bao-An CHEN ; De-Long LIU
Journal of Experimental Hematology 2008;16(5):1247-1250
DNA methylation is a frequent change in epigenetics of leukemia. In the development of leukemia, methylation plays an important role in the genetic expression regulation and genetic structure stabilization. Hematopoietic stem cell transplantation (HSCT) and chemotherapy are the common methods to cure leukemia at present. Unfortunately, it is very hard to find suitable donor for transplantation, while the relapse and drug resistance are the unresolved problems in chemotherapy. Because of the reversibility of methylation, the drugs reducing the level of methylation become a new way to cure leukemia. Decitabine is the most common medicament used to reduce the level of methylation. In this article, the methylation and tumor, methylation and hematologic diseases, methylation detection methods, demethylation therapy in leukemia and so on are reviewed.
DNA Methylation
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Humans
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Leukemia
3.Methylation and demethylation of DNA and histones in chromatin: the most complicated epigenetic marker.
Experimental & Molecular Medicine 2017;49(4):e321-
No abstract available.
Chromatin*
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DNA*
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Epigenomics*
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Histones*
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Methylation*
4.Current situation researching of methylation in tumor.
Di SHAO ; An-fang CUI ; Liu-luan ZHU ; Ai-jun QIAO ; Xing-xing KONG ; Xiao-jun LIU ; Yong-sheng CHANG ; Fu-de FANG
Acta Academiae Medicinae Sinicae 2009;31(6):786-790
The disorders of DNA and histone methylation have a close relationship with the development and progression of tumors. Epigenetic regulation is critical in maintaining the stability and integrity of the expression profiles of different cell types by modifying DNA methylation and histone methylation. However, the abnormal changes of methylation often result in the development and progression of tumors. This review summarized the theory of tumor genomic and histone methylation, detection methods of methylation and their applications, and the clinical application of methylation as biological markers and drug targets.
DNA Methylation
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Histones
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metabolism
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Humans
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Methylation
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Neoplasms
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genetics
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metabolism
5.Advances of research on demethylation therapy for hematologic malignancies.
Journal of Experimental Hematology 2009;17(5):1394-1398
DNA methylation is an important and reversible epigenetic modification which regulates genomic stability. Methylation is essential for mammalian development. Generally, gene expression level and DNA methylation are negative correlation. Transcriptional silencing via methylation of CpG islands in the promoter is important for cell growth and differentiation and plays a key role in tumorigenesis. Demethylation drug can modify chromatin and restore the ability of anti-oncogene. Demethylation therapy as a new therapy may treat efficiently hematological malignancies with resistance and relapse. In this review, DNA methylation mechanism, relationship between aberrant methylation and hematologic malignancies, mechanism of demethylation therapy, the advance of research on the demethylation therapy of hematological malignancies, such as acute and chronic leukemia, lymphoma, myelodysplastic syndrome were summarized.
DNA Methylation
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Hematologic Neoplasms
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genetics
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metabolism
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therapy
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Humans
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Methylation
6.Research progress and stress-induced variation of DNA methylation in plants.
Zhen YANG ; Xuanming PENG ; Yiyan ZHANG ; Yuanhai ZHANG ; Yong ZHANG
Chinese Journal of Biotechnology 2016;32(12):1642-1653
Chemical modification of DNA bases in recent years has been one of the hot areas of life science research. DNA methylation is a common epigenetic phenomenon and can change the genetic performance without changing the DNA sequence. Various stress factors can induce the variation of DNA methylation in plants, but the response mechanism is still unknown. In this paper, the progress of DNA methylation in plants was reviewed. In combination with the researchconclusions of our own research group, the DNA methylation variation induced by 7Li ion beam and gamma ray was reported to provide a basis for DNA methylation, which may be involved in the phenotypic plasticity of plants.
DNA Methylation
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Epigenesis, Genetic
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Epigenomics
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Plants
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genetics
7.Research Progress of m6A-Methylation in Acute Leukemia-Review.
Qiu-Ni CHEN ; Yue CHEN ; Ting-Ting JI ; Liang YU
Journal of Experimental Hematology 2019;27(6):2014-2018
Epigenetic abnormalities play an important role in the pathogenesis of hematological malignancies, especially acute leukemia (AL). Similar to DNA methylation and histone modifications, RNA methylation is another important epigenetic modification. m6A methylation is one of the most prevalent and extensively studied RNA methylation. m6A methylation is involved in many biological and pathological process. Recent studies have found that m6A methylation is involved in the occurrence, development and drug-resistance of AL. This review focuses on the research progress of m6A methylation in AL.
DNA Methylation
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Epigenesis, Genetic
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Humans
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Leukemia
8.Research Progress on Human Age Estimation Based on DNA Methylation.
Journal of Forensic Medicine 2020;36(1):109-114
In forensic science practice, age is an important individual information, and one of the indicators to be considered first to depict features of the suspect. Recently, DNA methylation has become a research hotspot in age estimation because of its hig accuracy and stability. New progress has been made in specificity of DNA methylation sites, age estimation in multiple tissues, DNA methylation age estimation of minors, sensitivity of age estimation, forensic practical applications, etc. At the same time, several studies also established more accurate statistical modeling methods, eliminated differences between different detection platforms, found appropriate number of sites in models and analyzed the influence of environment and diseases. This review summarizes these to provide references.
CpG Islands
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DNA Methylation
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Forensic Genetics
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Humans
9.DNA Methylation Differences in Peripheral Blood of Patients with Anaphylaxis.
Ming Zhe LIU ; Hua Lin GUO ; Yan FENG ; Jian Guo LI ; Peng Fei LI ; Cai Rong GAO ; Xiang Jie GUO
Journal of Forensic Medicine 2021;37(2):211-214
Objective To study the DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and to provide a new research direction and basis for the forensic diagnosis of shock caused by drug hypersensitiveness. Methods Methylation microarray was used to detect DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and normal subjects. Sequencing data and chip data were analyzed for differences in DNA methylation using R language methylkit, ChAMP package. Random forest algorithm was used to evaluate the importance of the DNA methylation differential sites. Results Differential sites of DNA methylation highly associated with anaphylaxis caused by cephalosporin drugs were obtained at loci such as ETS1, PRR23B and GNAS. Conclusion Cephalosporin allergy is associated with DNA methylation, and DNA methylation may be a new strategy for forensic identification of anaphylactic shock and death.
Anaphylaxis/genetics*
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DNA Methylation
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Forensic Medicine
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Humans
10.Classification of Colon Cancer Patients Based on the Methylation Patterns of Promoters.
Wonyoung CHOI ; Jungwoo LEE ; Jin Young LEE ; Sun Min LEE ; Da Won KIM ; Young Joon KIM
Genomics & Informatics 2016;14(2):46-52
Diverse somatic mutations have been reported to serve as cancer drivers. Recently, it has also been reported that epigenetic regulation is closely related to cancer development. However, the effect of epigenetic changes on cancer is still elusive. In this study, we analyzed DNA methylation data on colon cancer taken from The Caner Genome Atlas. We found that several promoters were significantly hypermethylated in colon cancer patients. Through clustering analysis of differentially methylated DNA regions, we were able to define subgroups of patients and observed clinical features associated with each subgroup. In addition, we analyzed the functional ontology of aberrantly methylated genes and identified the G-protein-coupled receptor signaling pathway as one of the major pathways affected epigenetically. In conclusion, our analysis shows the possibility of characterizing the clinical features of colon cancer subgroups based on DNA methylation patterns and provides lists of important genes and pathways possibly involved in colon cancer development.
Classification*
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Colon*
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Colonic Neoplasms*
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CpG Islands
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DNA
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DNA Methylation
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Epigenomics
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Genome
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Humans
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Methylation*