Occlusion or stenosis of lacrimal punctum may be from congenital origin, but can be caused by a variety of factors such as inflamations, tumors, traumas, and systemic diseases that invade punctum. Occlusion or stenosis of punctum was reported after use of drugs, such as strong miotics or idoxuridine also. The initial procedure to be employed in punctal stenosis caused by most factors is punctal dilation. In 1985, Awan used a new application of laser in the treatment of punctal stenosis, reporting good result. Two Korean adults with acquired punctal stenosis were treated by laser punctoplasty with good results.
Adult ; Constriction, Pathologic* ; Humans ; Idoxuridine ; Miotics

Occlusion or stenosis of lacrimal punctum may be from congenital origin, but can be caused by a variety of factors such as inflamations, tumors, traumas, and systemic diseases that invade punctum. Occlusion or stenosis of punctum was reported after use of drugs, such as strong miotics or idoxuridine also. The initial procedure to be employed in punctal stenosis caused by most factors is punctal dilation. In 1985, Awan used a new application of laser in the treatment of punctal stenosis, reporting good result. Two Korean adults with acquired punctal stenosis were treated by laser punctoplasty with good results.
Adult ; Constriction, Pathologic* ; Humans ; Idoxuridine ; Miotics

A study on 70 thyroid cancer patients at the K Hospital and Bach Mai Hospital got the results: thyroid cancer in females was 3.37 times more than in males. Mean age was 38.813.3 years. Incidence of distance metastases was 5/70 (7.1%). Histology type: Papillary cell carcinoma was 68.6%, follicular cell carcinoma is 12.8% and mixed papillary-follicular was 18.6%. Incidence of hypothyroidism was 100% (70/70) after 4 – 6 weeks total thyroidectomy. Thyroid remnants after total thyroidectomy was 5.253.01ml. Thyroid uptake of I-131 before treatment was 6.85.2%. The median serum concentration of thymoglobulin was 105.289.3 mg/ml
Thyroid Neoplasms, Neoplasm Metastasis, Surgery, Idoxuridine

PURPOSE: 5-iododeoxyuridine analogues have been exclusively developed for the potential antiviral and antitumor therapeutic agents. In this study, we synthesized carbocyclic radioiododeoxyuridineanalogue (ddIVDU) and carbocyclic intermediate as efficient carbocyclic radiopharmaceuticals. MATERIALS AND METHODS: The synthesis is LAH reduction, hetero Diels-Alder reaction as key reactions including Pd(0)-catalyzed coupling reaction together with organotin. MCA-RH7777 (MCA) and MCA-tk (HSV1-tk positive) cells were treated with various concentration of carbocyclic ddIVDU, and GCV. Cytotoxicity was measured by the MTS methods. For in vitro uptake study, MCA and MCA-tk cells were incubated with 1uCi of [(125)I]carbocyclic ddIVDU. Accumulated radioactivity was measured after various incubation times. RESULTS: The synthesis of ddIVDU and precursor for radioiodination were achieved from cyclopentadiene in good overall yield, respectively. The radioiododemetallation for radiolabeling gave more than 80% yield with > 95% radiochemical purity. GCV was more toxic than carbocyclic ddIVDU in MCA-tk cells. Accumulation of [(125)I]carbocyclic ddIVDU was higher in MCA-tk cells than MCA cells. CONCLUSION: Biological data reveal that ddIVDU is stable in vitro, less toxic than ganciclovir (GCV), and selective in HSV1-tk expressed cells. Thus, this new carbocyclic nucleoside, referred to in this paper as carbocyclic 2',3'-didehydro-2',3'-dideoxy-5- iodovinyluridine (carbocyclic ddIVDU), is a potential imaging probe for HSV1-tk.
Cycloaddition Reaction ; Ganciclovir ; Herpesvirus 1, Human ; Idoxuridine ; Radioactivity ; Radiopharmaceuticals

Acetyl salicylic acid (ASA) is metabolized by UDP-glucuronosyltransferase 1A6 (UGT1A6), cytochrome P4502C9 (CYP2C9), and N-acetyl transferase 2 (NAT2). Variations in the activities of these enzymes may modulate adverse ASA-related symptoms such as urticaria. We examined whether polymorphisms in the UGT1A6, CYP2C9, and NAT2 genes are related to ASA-intolerant urticaria (AIU). The genotypes of 148 subjects with AIU (AIU group) and 260 normal healthy control subjects (NC group) were analyzed with respect to the following single nucleotide polymorphisms: CYP2C9 -1188T>C and CYP2C9*3A1075C; UGT1A6 T181A A>G and UGT1A6 R184S A>C; and NAT2 9796A>T, NAT2 197G>A, NAT2 286G>A, NAT2 9601A>G, and NAT2 9306A>G. There were significant differences in the allele frequencies for the CYP2C9 polymorphisms between the two groups. The frequency of the minor allele CYP2C9 -1188T>C was significantly higher in the AIU group than in the NC group (P=0.005). The frequency of the variant genotype CC was higher in the AIU group compared with the controls in both the co-dominant (P=0.007) and recessive models (P=0.012). The frequency of haplotype 2 [CA] was also significantly higher in the AIU group in both the co-dominant (P=0.006) and dominant models (P=0.012). There was no significant difference in genotype frequencies for any of the UGT1A6 or NAT2 polymorphisms between the two groups. Clinical parameters did not differ according to genotype. These results suggest that the C allele of CYP2C9 -1188T>C may be associated with AIU.
Alleles ; Aspirin ; Cytochromes ; Gene Frequency ; Genotype ; Haplotypes ; Idoxuridine ; Salicylic Acid ; Transferases ; Urticaria

The clinical and statistical review were carried out with 92 cases of herpetic keratitis at the Dept. of Ophthalmology, St. Mary's Hospital, Catholic Medical College from Jan. 1974 to Aug. 1976. The results were as follow: 1. Herpetic keratitis was most prevalent at the age of 30's and least at those under 10 and 60'. 2. Male and female ratio was about 3 : 1 but the ratio under the age of 20 was even. 3. The most epidemic season was from March to June, amounting to about 56.5% of the total, and exhaustion was the most significant predisposing factor. 4. The simple type of herpetic keratitis was 45 cases (48.9%) of the total while the complicated type was 47 cases(51.1%). Four fifth of simple type was dendritic keratitis with 36cases (39.1%) and disciform keratitis was most frequent among the complicated type with 20 cases (21.7%). 5. Involvement was even between left and right eyes. 80% of binocular cases was primarily involved at the age of over 20's. Among binocular cases, both eyes showed similar signs and course. 6. Vision in the early stage depends on the location of keratitis invloved. Twenty two out of twenty three cases with duration over 3 years displayed and obvious decreased vision less than 0.1 which implied that the longer the duration is, the more evident the disturbance in vIsion is. 7. In 30 cases the effect of 5-ido-2'-deoxyuridine (I.D.U.) was excellent while there was no effective response to the 5-iodo-2'-deoxyuridine (I.D.U.) in 29 cases. In cases resistant against I.D.U., cryotherapy and keratoplasty proved to be very effective.
Causality ; Corneal Transplantation ; Cryotherapy ; Female ; Humans ; Idoxuridine ; Keratitis* ; Keratitis, Dendritic ; Keratitis, Herpetic ; Male ; Ophthalmology ; Seasons ; Telescopes

PURPOSE: Hydrodynamic-based procedure is a simple and effective gene delivery method to lead a high gene expression in liver tissue. Non-invasive imaging reporter gene system has been used widely with herpes simplex virus type 1 thymidine kinase (HSV1-tk) and its various substrates. In the present study, we investigated to image the expression of HSV1-tk gene with 5-(2-iodovinyl)-2'-deoxyuridine (IVDU) in mouse liver by the hydrodynamicbased procedure. MATERIALS AND METHODS: HSV1-tk or enhanced green fluorescence protein (EGFP) encoded plasmid DNA was transferred into the mouse liver by hydrodynaminc injection. At 24 h post-injection, RT-PCR, biodistribution, fluorescence imaging, nuclear imaging and digital wholebody autoradiography (DWBA) were performed to confirm transferred gene expression. RESULTS: In RT-PCR assay using mRNA from the mouse liver, specific bands of HSV1-tk and EGFP gene were observed in HSV1-tk and EGFP expressing plasmid injected mouse, respectively. Higher uptake of radiolabeled IVDU was exhibited in liver of HSV1-tk gene transferred mouse by biodistribution study. In fluorescence imaging, the liver showed specific fluorescence signal in EGFP gene transferred mouse. Gamma-camera image and DWBA results showed that radiolabeled IVDU was accumulated in the liver of HSV1-tk gene transferred mouse. CONCLUSION: In this study, hydrodynamic-based procedure was effective in liver-specific gene delivery and it could be quantified with molecular imaging methods. Therefore, co-expression of HSV1-tk reporter gene and target gene by hydrodynamic-based procedure is expected to be a useful method for the evaluation of the target gene expression level with radiolabeled IVDU.
Animals ; Autoradiography ; DNA ; Fluorescence ; Gene Expression ; Genes, Reporter ; Herpes Simplex ; Herpesvirus 1, Human ; Idoxuridine ; Liver ; Methylmethacrylates ; Mice ; Molecular Imaging ; Optical Imaging ; Plasmids ; Polystyrenes ; RNA, Messenger ; Simplexvirus ; Thymidine Kinase

Tumor cell proliferation is considered to be a useful prognostic indicator of tumor aggressiveness and tumor response to therapy, but in vitro measurement of individual proliferation is complex and tedious work. PET imaging provides a noninvasive approach to measure tumor growth rate in situ. Early approaches have used 18F-FDG or methionine to monitor proliferation status. These 2 tracers detect changes in glucose and amino acid metabolism, respectively, and therefore provide only an indirect measure of proliferation status. More recent studies have focused on DNA synthesis itself as a marker of cell proliferation. Cell lines and tissues with a high proliferation rate require high rates of DNA synthesis. [11C]Thymidine was the first radiotracer for noninvasive imaging of tumor proliferation. The short half-life of 11C and rapid metabolism of [11C]thymidine in vivo make the radiotracer less suitable for routine use. Halogenated thymidine analogs such as 5-iodo-2-deoxyuridine (IUdR) can be successfully used as cell proliferation markers for in vitro studies because these compounds are rapidly incorporated into newly synthesized DNA. IUdR has been evaluated as a potential in vivo tracer in nuclear medicine, but the image quality and the calculation of proliferation rates are impaired by its rapid in vivo degradation. Hence, the thymidine analog 3'-deoxy-3'-18F-fluorothymidine (FLT) was recently introduced as a stable proliferation marker with a suitable nuclide half-life and stable in vivo. [18F]FLT is phosphorylated to 3-fluorothymidine monophosphate by thymidine kinase 1 and reflects thymidine kinase 1 activity in proliferating cell. [18F]FLT PET is feasible in clincal use and well correlates with cellular proliferation. Choline is a precursor for the biosynthesis of phospholipids (in particular, phosphatidylcholine), which is the essential component of all eukaryotic cell membranes and [11C]choline, which is a new marker for cellular proliferation.
Cell Line ; Cell Proliferation* ; Choline ; DNA ; Eukaryotic Cells ; Fluorodeoxyglucose F18 ; Glucose ; Half-Life ; Idoxuridine ; Membranes ; Metabolism ; Methionine ; Nuclear Medicine ; Phospholipids ; Thymidine ; Thymidine Kinase