1.Changes of hydroxyprolin in the slake lime burn treated by maduxin
Journal of Practical Medicine 2002;435(11):28-30
Maduxin ointment is totally extracts from the leaves of the Maduca pasquieri and has been applied in burn treatment in Vietnam since 1987. We studied the effects of Maduxin on the collagenization at burn wounds by following up the changes of OH-Pro content at slaked lime burn wounds - a popular type of burn in Vietnam in comparison with the effects of SSD in burn treatment. Our study was carried out in 21 slaked lime burn patients who were treated at the Vietnam Burn Institute from May to September 1997. The results indicated that OH-Pro concentration increases related to collagen synthesis during the burn wound healing. In the slaked lime burn wounds treated by Maduxin OH-Pro level in burn tissue increases more than in the wounds treated by Silvesufadiazin during the healing.
Hydroxyproline
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Therapeutics
2.Advances on microbial synthesis of L-proline and trans-4-hydroxy-L-proline.
Xiaolu HU ; Shumei CUI ; Chongrong KE ; Yong TAO ; Jianzhong HUANG ; Xinwei YANG
Chinese Journal of Biotechnology 2022;38(12):4498-4519
L-proline (L-Pro) is the only imino acid among the 20 amino acids that constitute biological proteins, and its main hydroxylated product is trans-4-hydroxy-L-proline (T-4-Hyp). Both of them have unique biological activities and play important roles in biomedicine, food and beauty industry. With the in-depth exploration of the functions of L-Pro and T-4-Hyp, the demand for them is gradually increasing. Traditional methods of biological extraction and chemical synthesis are unable to meet the demand of "green, environmental protection and high efficiency". In recent years, synthetic biology has developed rapidly. Through the intensive analysis of the synthetic pathways of L-Pro and T-4-Hyp, microbial cell factories were constructed for large-scale production, which opened a new chapter for the green and efficient production of L-Pro and T-4-Hyp. This paper reviews the application and production methods of L-Pro and T-4-Hyp, the metabolic pathways for microbial synthesis of L-Pro and T-4-Hyp, and the engineering strategies and advances on microbial production of L-Pro and T-4-Hyp, aiming to provide a theoretical basis for the "green bio-manufacturing" of L-Pro and T-4-Hyp and promote their industrial production.
Proline
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Hydroxyproline
3.Effects of Intake of Alcohol on the Concentration of Urinary 4-hydroxyproline.
Keou Weon LEE ; Kyung Jong LEE ; Jae Beom PARK ; Kyu Yeub JANG ; Chul Woo BANG ; Young Bong CHO
Korean Journal of Aerospace and Environmental Medicine 2003;13(3):144-148
BACKGROUND: We studied whether alcohol intake influences in urinary 4-Hyp concentration and whether 4-Hyp concentration is change by time in healthy office workers for 2 weeks. METHODS: Three healthy male workers, who has a same job, recruited for this study, aged from 29 to 33 years, who had been any diagnosed or treated to the alcohol or bone related diseases. Urine samples were picked about 30 ml in screw capped vials for 2 weeks, and analysing method were used to previous published method for determination of urinary 4-Hyp with dansyl chloride. RESULTS: From the ANOVA-test results about objects' Hyp concentration that classify by urine picking time, When subjects did not ingest alcohol, urinary 4-Hyp concentration was difference according to subjects for-work and post-work. But, did not show difference when subjects ingested alcohol. While, T-test result that do alcohol intake existence and nonexistence and urine picking time by variable, urinary 4-Hyp concentration did not show difference for-work and post-work, but there was difference in post-work that is next day that ingest alcohol. When did not ingest alcohol, mean concentration of urinary 4-Hyp increased go by post-work (3.80+/-6.47 nmol/mg creatinine) in pre-work (1.46+/-1.61 nmol/mg creatinine). But when ingested alcohol, mean concentration of urinary 4-Hyp decreased by the next day morning (3.32+/-6.36 nmol/mg creatinine), order of for-work (1.54+/-1.59 nmol/mg creatinine) in alcohol intake future generations post-work (5.09+/-6.41 nmol/mg creatinine). CONCLUSION: We confirmed that alcohol is influencing in urinary 4-Hyp concentration of three healthy male workers, who has a same job, for 2 weeks, and mean concentration of 4-Hyp increasing go by the post-work in the pre-work, could confirm that influence in the next day morning if ingest alcohol in the evening.
Humans
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Hydroxyproline*
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Male
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Social Responsibility
4.Experimental Studies on the Vitreous Substitutes.
Young Soo HAHN ; Won Shik YOUN
Journal of the Korean Ophthalmological Society 1973;14(4):309-314
Experimental replacements of rabbit vitreous by air, normal saline, dextran and Haemaccel were tried in albino rabbits. After aspiration of 0.7ml of liquid vitreous from the central portion of vitreous, 0.5ml of substitutes were injected. Clinically, these substitutes were well tolerated with slight uveal inflammation which subsided within one week. Intraocular pussure returned to normal value after 5 days. Hexosamine content of the whole vitreous samples showed moderate increase after Haemaccel injection which decreased slowly thereafter, whereas no significant changes were observed with other substitutes. Hydroxyproline content of the whole vitreous also showed marked increase after injection of Haemaccel, owing to the high hydroxyproline content of HaemacceJ. After 6 weeks 22.9% of the injected hydroxyproline remained in the vitreous. With other substitutes no change was observed.
Dextrans
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Hydroxyproline
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Inflammation
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Polygeline
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Rabbits
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Reference Values
5.Coordination mechanism and antioxidant activity of L-hydroxyproline zinc(II).
Rui-Ya ZUO ; Xiao-Hua ZHOU ; Shou-Ying DU
Chinese Journal of Biotechnology 2007;23(4):704-709
The coordination compound of L-hydroxyproline (Hyp)-Zn (II) was synthesized with Hyp and zinc sulfate as raw materials in water medium, coordination Synthesizing Mechanism and Antioxidant Activity of Hyp-Zn(II) coordination compound has been researched. Compared with Hyp, the infrared spectrogram of Hyp-Zn (II) coordination compound emerge a new absorption peak at 1100 cm(-1). Conclusion could be obtained that there exists a coordination effect between Hyp and ZnSO4; TG and DSC curve of Hyp and Hyp-Zn(II) coordination compound were analysed. Compared with Hyp, the peak of Hyp-Zn(II) disappear at 290 degrees C and 375 degrees C. This phenomenon confirmed the front conclusion; At the NMR graph of Hyp-Zn(II) coordination compound, the disappearance of the alpha-carboxyl-hydrogen and alpha-hydroxyl-hydrogen's peak at 3.5-3.9 ppm could indicate that combination's position of Hyp is alpha-carboxyl and alpha-hydroxyl; Structure of Hyp-Zn(II) coordination compound were exosyndrome by the Atomic Force microscopy. It is showed that centr-atom Zn(II) was surrounded by several Hyp at Hyp-Zn(II) coordination compound's phase diagram; The proportion of Hyp-Zn(H) coordination compound was determined by dialysis experiment, the proportion is 4:1; Above-mentioned determination and exosyndrome indicated that the molecular formula of Hyp-Zn (II) coordination compound is Zn(Hyp)4.H2O. The results indicated that the Hyp-Zn(II) coordination compound can inhibit hydroxyl free radicals of Zn(II), and the Percentage of Inhibition is 75.5%; the total antioxidant activities of Hyp-Zn(II) coordination compound is 80.167 u/mL, the anti-superoxide activities of Hyp-Zn (II) coordination compound is 53.19 u/mL.
Antioxidants
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chemistry
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Drug Compounding
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Hydroxyproline
;
chemistry
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Zinc Sulfate
;
chemistry
6.Study on hydroxyproline tolerance of different cultivars of Chrysanthemum morifolium in vitro.
Yanfang ZHU ; Zhaoyang GUO ; Jianping XUE ; Aimin ZHANG ; Wei SHENG ; Yunxian SONG
China Journal of Chinese Materia Medica 2011;36(19):2625-2628
OBJECTIVETo investigate hydroxyproline tolerance of Chrysanthemum morifolium plantlets included " Boju", "Huaiju", "Chuju", "Gongju" and "Hangju",and provide references basis for excellent cultivar and breeding of Ch. morifolium.
METHODPlantlets in vitro from five kinds of Ch. morifolium were inoculated on medium added with different concentrations of hydroxyproline. Free proline in leaves from plantlets was determined, then the damage index and survival rate were compared.
RESULT AND CONCLUSIONThe results showed that hydroxyproline tolerance of " Boju" and "Huaiju" were superior, the survival rates and free proline of them were higher, but the damage index was inferior. The hydroxyproline tolerance of "Hangju" was the worst, and the survival rate was minimum. The survival rate of "Chuju" and "Gongju" was between "Boju" and " Hangju", and the hydroxyproline tolerance of them was also medium.
Chrysanthemum ; chemistry ; classification ; metabolism ; Culture Techniques ; Hydroxyproline ; analysis ; metabolism
7.The effects of ventilation with high density oxygen on the strength of gastrointestinal anastomosis.
Tevfik EKER ; Volkan GENC ; Yusuf SEVIM ; Ozge CUMAOGULLARI ; Menekse OZCELIK ; Akin Firat KOCAAY ; Cemal Ozben ENSARI ; Ozge Tugce PASAOGLU
Annals of Surgical Treatment and Research 2015;89(1):17-22
PURPOSE: The aim of our study is to evaluate the effects of administration of perioperative supplemental oxygen on anastomoses. METHODS: Forty male Wistar albino rats were used in the study and randomized into 4 groups. Ischemia-reperfusion models were built in groups 3 and 4. Jejunojejunostomy was performed in all rats and assigned to an oxygen/nitrous oxide mixture with a fraction of inspired oxygen of 30% in groups 1 and 3 and 80% in groups 2 and 4. The measurements of perianastomotic tissue oxygen pressure, bursting pressure, level of hydroxyproline were evaluated and compared in all groups. RESULTS: The perianastomotic tissue oxygen pressures, bursting pressures and levels of hydroxyproline were identified as significantly high in groups 2 and 4, administered a fraction of inspired oxygen of 80%, compared to groups 1 and 3, administered a fraction of inspired oxygen of 30%. CONCLUSION: Perioperative supplemental oxygen contributes positively to the anastomotic healing.
Animals
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Humans
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Hydroxyproline
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Male
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Oxygen*
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Rats
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Rats, Wistar
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Ventilation*
8.An Analysis on the Priority of Selection of Supplier for School Food Service Materials.
Myung Joo PARK ; Suk Whan KIM ; Joung Sil LEE
Korean Journal of Community Nutrition 2008;13(4):531-539
The purpose of this study is to examine how nutritionists, principals and parents evaluated the importance and priorities of different selection factors for food materials suppliers in an attempt to suggest how to ensure the best supplier choice, since the selection of the best supplier was mandatory for the successful TQM of school food services. This study especially aims to develop priority alternatives based on relative rather than absolute assignments on selection of suppliers for school food service materials. To apply AHP, the selection factors of a food materials suppliers were grouped into five categories, which included quality, service, reliability, hygiene and price. And the five categories involved 28 elements. As the result, quality was considered most crucial on the whole, followed by service, reliability, hygiene and price; and food labeling in the category of quality evaluated as that of first priority in the totality evaluation elements.
Dietary Sucrose
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Food Labeling
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Food Services
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Humans
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Hydroxyproline
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Hygiene
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Parents
9.Experimental research of stent anastomosis of gastrojejunostomy in a porcine model.
Xiu-Jun CAI ; Yi-Chen YU ; Hua-Jie CAI ; Yi-Fan WANG
Chinese Medical Journal 2011;124(3):408-412
BACKGROUNDGastrojejunostomy is one of the most frequently used procedures for general surgeons. The creation of anastomosis between various parts of the gastrointestinal tract is a basic technical component and major task in the daily practice of almost all gastrointestinal procedures. This research evaluated a new procedure of making gastrointestinal anastomosis with stent.
METHODSTwenty experimental mini-pigs were randomized into two groups. In stent anastomosis group (SA), the anastomoses were constructed with a poly-levolactic acid stent. In hand-sewn group (HA), the anastomoses were performed with a single-layer continuous suture. Abdominal X-ray with intraluminal contrast was performed on the 10th postoperative day. Five pigs of each group were sacrificed on the postoperative days 3 and 14 to determine anastomotic bursting pressure in situ, hydroxyproline concentration, and histopathological evaluation of the anastomotic sites.
RESULTSThere was no intraoperative morbidity or mortality. The median time needed for the sutured anastomosis was (21.7 ± 2.3) minutes and for the stent anastomosis was (11.9 ± 1.9) minutes (P < 0.001). Abdominal X-ray with intraluminal contrast demonstrated normal gas distribution and showed no evidence of leakage or obstruction. Macroscopic appearance at the longitudinal opening of anastomosis was always good in both groups. The median anastomotic bursting pressure was (18.2 ± 1.6) kPa in SA group on postoperative day 3, compared with (11.7 ± 3.2) kPa in HA group (P = 0.003). The anastomotic bursting pressure on day 14 was not significantly different between SA group ((27.1 ± 2.6) kPa) and HA group ((28.3 ± 1.7) kPa) (P = 0.388). The hydroxyproline concentrations were not significantly different.
CONCLUSIONSThe stent anastomosis was not considered to be more difficult than a sutured anastomosis. This method is proved to be safe and feasible compared with the traditional hand-sewn method in the porcine model. The method increases early anastomotic strength in this study.
Anastomosis, Surgical ; methods ; Animals ; Female ; Gastric Bypass ; methods ; Hydroxyproline ; metabolism ; Male ; Stents ; Swine
10.Determination of hydroxyproline in rat kidney tissue by HPLC with pre-column derivatization.
Er-gang LIU ; Xu-chun FU ; Ying QIAN ; Hai-bo BAI
Journal of Zhejiang University. Medical sciences 2012;41(2):188-191
OBJECTIVETo establish a pre-column derivatization HPLC method for quantitation of hydroxyproline (Hyp) in rat kidney tissue by reversed-phase assay.
METHODSRat kidney samples were hydrolyzed in 6 mol x L⁻¹ HCl under 110 degree for 24 h, then 9-fluorenylmethyl chloroformate (FMOC) was added for the pre-column reaction. The HPLC analysis was performed on a Phenomenex C₁₈ column using sodium acetate buffer, methanol and acetonitrile as mobile phase with a flow rate of 1.0 ml min⁻¹ and UV detective wavelength 265 nm at 40 degree.
RESULTSLinear range was 15.30-612.00 mg x L⁻¹ (correlation coefficient was 0.9999). Recovery rate was 97.4%-103.9%.
CONCLUSIONThe established method is simple, accurate and sensitive to analyze Hyp content in rat kidney tissue.
Animals ; Chromatography, High Pressure Liquid ; methods ; Hydroxyproline ; analysis ; In Vitro Techniques ; Kidney ; chemistry ; Male ; Rats