No abstract available.
Hemolysis*

BACKGROUND: Sometimes we receive specimens collected several hours ahead, hemolyzed, or turbid. To decide to analyze the samples or not, the influence of storage time, hemolysis, and turbidi-ty on PT and PTT were studied. METHODS: Total of 198 cases for PT and 192 for aPTT were studied to clarify the effect of storage time on PT and aPTT. We performed the tests of PT and aPTT at 0, 2, 4, and 8 hours after collection of specimens. For the hemolysis effect study, we used 31 cases. Slightly, moderately, and severely hemolyzed plasma were obtained by adding hemolysate to plasma. For the turbidity effect study, we used 30 cases. Slightly, moderately, and severely turbid plasma were obtained by adding Intrali-pos (20% soybean oil) to plasma. RESULTS: Delayed test time affected the aPTT results after 8 hours. Hemolysis affected the PT and aPTT results. Turbidity did not affect the PT and aPTT results; however, the Coagrex-100s rejected to analyze the severely turbid samples. CONCLUSIONS: It is recommended that specimens be evaluated immediately or within 4 hours and that hemolysed or severely turbid specimens for PT and aPTT tests not be used.
Hemolysis* ; Plasma ; Soybeans

During massive transfusions especially under pressure, considerable hemolysis has been expected. It had been assumed that hernolysis would increase with increasing age of the bank blood, increasing pressure and with decreasing bore of the needle. Moss and Stauntan, however, found that hemolysis actually increased when blood was forced through larger bore needles. The authors have studied the magnitude of hemolysis according to various needle sizes under ordinary clinical transfusion conditions, and concluded as follows: 1) Hemolysis was maximum when a 18G needle was used and minimum with use of a 22G. needle regardless of the age and temperature of the bank blood, 2) Hemolysis increased with increasing age of the bank blood. 3) When the needle size was constant, hemolysis was not affected by warming of the bank blood.
Clinical Study* ; Hemolysis* ; Needles

It is very common that stored blood and various replacement solutions are simultaneously administrated during operation. The stored blood of healthy man was used to find out the hemolysis due to various replacemen solutions for 21 days. We used for the test solutions which were 5% Dextrose in Water, 5% Dextrose and Saline, N-Saline, Volex, Rheomacrodex-D and Hartmann's solution. The results are as follows: 1) It was very significant that F-Value of all the replacement solutions was 8,4773. 2) The most prominent effect of hemolysis was in Rhemacrodex-D and the least one was hartmann's solution among the six replacement solutions for 21 days. 3) It was shown the peaked hemolysis aroun 15th day in the six replacement solutions.
Glucose ; Hemolysis* ; Water

No abstract available.
Epilepsia Partialis Continua ; Hemolysis ; Midazolam

BACKGROUND: The selection of identification (ID) system of enterococci depends mainly on the accuracy of ID system, cost of operation and convenience of testing. Commercial ID kits are easy to use but too expensive. The aim of the study was to develop a simple system for the identification of species of enterococci which are frequently isolated from clinical specimens. METHODS: Eight conventional biochemical tests selected for the simple ID method were hemolysis pattern, NaCl-esculin hydrolysis, tellurite tolerance, arginine dihydrolase, acid from arabinose, raffinose and methyl-alpha-D-glucopyranoside, and pigment production. Ninety one consecutive strains of enterococci from clinical specimens isolated during the period of April 1988 were tested by the simple ID method, and API rapid ID 32 STREP. RESULTS: Simple ID method with 15 ID codes was established to identify 13 species of enterococci. Among the 91 isolates tested, 88 (96.7%) were identified to the species level of enterococci by simple ID method. CONCLUSIONS: The simplified conventional ID method is simple, reliable and economical. Further modification may improve the accuracy of the enterococcal species identification system.
Arabinose ; Arginine ; Hemolysis ; Hydrolysis ; Raffinose

Transcatheter occlusion of the patent ductus arteriosus(PDA) using the Rashkind double umbrella device has become an accepted practice as an effective alternative to surgical ligation in many centers. We experienced three cases of medically refractory severe mechanical hemolysis following transcatheter closure of PDA due to residual shunt. Hemolysis resolved rapidly and completely after deployment of second or more Rashkind umbrellas without surgical correction of the residual shunt. In conclusion, reinsertion of the Rashkind umbrella is a useful modality for treatment of severe hemolysis developed after transcatheter closure of the PDA.
Ductus Arteriosus, Patent* ; Hemolysis* ; Ligation

100 patients (54 in U.B hospital, 46 in Pitea) underwent TURP using a solution 2% ethanol as irrigating water; an expired breath alcohol meter was used2 to monitor ethanol in the patients breath every 5 min. P-fH6 was assessed before and after TURP in 99 patients. Other markers of hemolysis were also evaluated in the Swedish group. Result: 32 patients had detectable ethanol in their breath. There was a close correlation between the maximum ethanol reading during surgery and the level of p-fHb after TURP (r=0.90, p<0.001).
Hemolysis ; Transurethral Resection of Prostate ; surgery

Severe hemolysis occurred after transcatheter occlusion of a patent ductus arteriosus in 3 patients among 41 patients who underwent transcatheter occlusion of a patent ductus arteriosus. The problems were managed by a standard surgical ligation of the duct, leaving the occlusion device(the Rashkind umbrella and a detachable coil) in situ in one patient, surgical removal of the detachable coil and division of the duct in another and abated with supportive care in the other.
Ductus Arteriosus, Patent* ; Hemolysis* ; Humans ; Ligation

Severe hemolysis occurred after transcatheter occlusion of a patent ductus arteriosus in 3 patients among 41 patients who underwent transcatheter occlusion of a patent ductus arteriosus. The problems were managed by a standard surgical ligation of the duct, leaving the occlusion device(the Rashkind umbrella and a detachable coil) in situ in one patient, surgical removal of the detachable coil and division of the duct in another and abated with supportive care in the other.
Ductus Arteriosus, Patent* ; Hemolysis* ; Humans ; Ligation